Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14538 and 14539 are separated by 100 nucleotides14539 and 14540 are separated by 433 nucleotides14540 and 14541 are separated by 369 nucleotides14541 and 14542 are separated by 587 nucleotides mRNA_6170: 14538 - KOG3476 Microtubule-associated protein CRIPT, at 165,822 to 166,606 14538 mRNA_6171: 14539 - SUPERFAMILY--SSF52047, at 166,707 to 169,416 14539 mRNA_6172: 14540 - K02685 PRI2 DNA primase large subunit, at 169,850 to 171,909 14540 mRNA_6173: 14541 - K00698 CHS1 chitin synthase, at 172,279 to 176,176 14541 mRNA_6174: 14542 - BLAST Glycoside hydrolase [Aureobasidium pullulans], at 176,764 to 178,095 14542
Group Condition mRNA_6170 mRNA_6171 mRNA_6172 mRNA_6173 mRNA_6174
14538 14539 14540 14541 14542
growth on ricinoleic acid YNB Ricinoleic Acid -0.8 -0.2 N.D. -0.3 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.8 -0.0 N.D. -0.0 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 -0.5 N.D. +0.3 -1.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 +0.1 N.D. -0.0 -0.6
auxotrophy YNB Glucose 75mM Arginine -0.6 -0.2 N.D. +0.2 +0.1
auxotrophy Dropout Complete -0.7 -0.1 N.D. +0.2 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.0 N.D. -0.2 +0.1
growth on oleic acid YNB Oleic Acid -0.1 +0.0 N.D. +0.0 -0.2
auxotrophy YNB Glucose -0.4 -0.3 N.D. +0.1 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 -0.4 N.D. +0.1 -0.2
growth on oleic acid YNB Glucose -0.1 +0.1 N.D. +0.0 -0.2
growth on ricinoleic acid YNB Glucose -0.2 +0.2 N.D. -0.2 +0.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.1 N.D. +0.1 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 +0.1 N.D. +0.2 -0.3
growth on ypd YPD -0.4 +0.4 N.D. +0.3 -0.3
auxotrophy YNB Glucose 75mM Methionine -0.6 +0.1 N.D. +0.2 +0.3
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.1 N.D. +0.1 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.3 -0.2 N.D. +0.1 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 +0.2 N.D. +0.4 -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.2 +0.2 N.D. +0.2 +0.4
facs on lipid accumulation Sorted No BODIPY Gate +0.4 +0.1 N.D. +0.2 +0.4
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