Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14539 and 14540 are separated by 433 nucleotides14540 and 14541 are separated by 369 nucleotides14541 and 14542 are separated by 587 nucleotides14542 and 14543 are separated by 261 nucleotides mRNA_6171: 14539 - SUPERFAMILY--SSF52047, at 166,707 to 169,416 14539 mRNA_6172: 14540 - K02685 PRI2 DNA primase large subunit, at 169,850 to 171,909 14540 mRNA_6173: 14541 - K00698 CHS1 chitin synthase, at 172,279 to 176,176 14541 mRNA_6174: 14542 - BLAST Glycoside hydrolase [Aureobasidium pullulans], at 176,764 to 178,095 14542 mRNA_6175: 14543 - KOG0255 Synaptic vesicle transporter SVOP and related transporters (major facilitator superfamily), at 178,357 to 180,910 14543
Group Condition mRNA_6171 mRNA_6172 mRNA_6173 mRNA_6174 mRNA_6175
14539 14540 14541 14542 14543
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 N.D. +0.3 -1.3 -0.9
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 N.D. +0.1 -0.2 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 N.D. -0.2 +0.1 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. -0.0 -0.6 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 N.D. -0.0 -0.3 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 N.D. -0.3 +0.1 +0.1
growth on oleic acid YNB Glucose +0.1 N.D. +0.0 -0.2 -0.1
growth on oleic acid YNB Oleic Acid +0.0 N.D. +0.0 -0.2 +0.1
growth on ricinoleic acid YNB Glucose +0.2 N.D. -0.2 +0.2 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. +0.1 -0.1 +0.0
auxotrophy Dropout Complete -0.1 N.D. +0.2 +0.0 -0.0
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. +0.2 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. +0.2 -0.3 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.1 +0.1 -0.0
auxotrophy YNB Glucose -0.3 N.D. +0.1 +0.4 +0.2
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. +0.1 +0.0 +0.5
growth on ypd YPD +0.4 N.D. +0.3 -0.3 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. +0.2 +0.3 -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.2 N.D. +0.2 +0.4 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. +0.2 +0.4 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. +0.4 -0.1 +0.4
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