Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14540 and 14541 are separated by 369 nucleotides14541 and 14542 are separated by 587 nucleotides14542 and 14543 are separated by 261 nucleotides14543 and 14544 are separated by 1415 nucleotides mRNA_6172: 14540 - K02685 PRI2 DNA primase large subunit, at 169,850 to 171,909 14540 mRNA_6173: 14541 - K00698 CHS1 chitin synthase, at 172,279 to 176,176 14541 mRNA_6174: 14542 - BLAST Glycoside hydrolase [Aureobasidium pullulans], at 176,764 to 178,095 14542 mRNA_6175: 14543 - KOG0255 Synaptic vesicle transporter SVOP and related transporters (major facilitator superfamily), at 178,357 to 180,910 14543 mRNA_6176: 14544 - K03000 RPA12, ZNRD1 DNA-directed RNA polymerase I subunit RPA12, at 182,326 to 182,954 14544
Group Condition mRNA_6172 mRNA_6173 mRNA_6174 mRNA_6175 mRNA_6176
14540 14541 14542 14543 14544
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.3 -1.3 -0.9 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.0 -0.6 +0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.2 +0.1 -0.3 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.0 -0.3 -0.1 N.D.
growth on oleic acid YNB Glucose N.D. +0.0 -0.2 -0.1 N.D.
growth on ricinoleic acid YNB Glucose N.D. -0.2 +0.2 -0.2 N.D.
growth on oleic acid YNB Oleic Acid N.D. +0.0 -0.2 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 -0.2 +0.0 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.3 +0.1 +0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 -0.1 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.2 -0.3 +0.2 N.D.
growth on ypd YPD N.D. +0.3 -0.3 +0.1 N.D.
auxotrophy Dropout Complete N.D. +0.2 +0.0 -0.0 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 +0.1 -0.0 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 +0.1 +0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 +0.3 -0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.2 +0.4 -0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 +0.0 +0.5 N.D.
auxotrophy YNB Glucose N.D. +0.1 +0.4 +0.2 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.4 -0.1 +0.4 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.2 +0.4 +0.2 N.D.
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