Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14544 and 14545 are separated by 379 nucleotides14545 and 14546 are separated by 993 nucleotides14546 and 14547 are separated by 378 nucleotides14547 and 14548 are separated by 112 nucleotides mRNA_6176: 14544 - K03000 RPA12, ZNRD1 DNA-directed RNA polymerase I subunit RPA12, at 182,326 to 182,954 14544 mRNA_6177: 14545 - K00868 pdxK, pdxY pyridoxine kinase, at 183,334 to 184,793 14545 mRNA_6178: 14546 - K17623 HDHD1 pseudouridine 5'-phosphatase, at 185,787 to 186,902 14546 mRNA_6179: 14547 - KOG2101 Intermediate filament-like protein, sorting nexins, and related proteins containing PX (PhoX) domain(s), at 187,281 to 188,756 14547 mRNA_6180: 14548 - KOG0218 Mismatch repair MSH3, at 188,869 to 192,529 14548
Group Condition mRNA_6176 mRNA_6177 mRNA_6178 mRNA_6179 mRNA_6180
14544 14545 14546 14547 14548
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.6 -0.2 -1.3 -1.7
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.4 -0.1 -0.6 -1.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 +0.0 -0.9 -1.2
growth on ypd YPD N.D. -0.2 +0.1 -1.1 -0.4
growth on oleic acid YNB Glucose N.D. -0.2 -0.2 -0.3 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.0 -0.1 -0.8 +0.2
growth on ricinoleic acid YNB Glucose N.D. +0.5 -0.2 -0.3 -0.7
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 -0.2 -0.0 -0.7
auxotrophy YNB Glucose N.D. +0.2 -0.2 -0.3 -0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 -0.1 -0.4 +0.0
auxotrophy Dropout Complete N.D. +0.2 -0.2 +0.0 -0.3
growth on oleic acid YNB Oleic Acid N.D. +0.1 -0.1 -0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.3 -0.0 -0.5 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.6 -0.3 +0.4 -0.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.2 -0.3 +0.3 +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. -0.0 +0.0 +0.0 -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 +0.2 -0.0 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.0 -0.1 -0.0 +0.3
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 +0.1 -0.7 +0.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 -0.1 +0.9 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.5 -0.1 +0.7 -0.2
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