Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14547 and 14548 are separated by 112 nucleotides14548 and 14549 are separated by 456 nucleotides14549 and 14550 are separated by 796 nucleotides14550 and 14551 are separated by 658 nucleotides mRNA_6179: 14547 - KOG2101 Intermediate filament-like protein, sorting nexins, and related proteins containing PX (PhoX) domain(s), at 187,281 to 188,756 14547 mRNA_6180: 14548 - KOG0218 Mismatch repair MSH3, at 188,869 to 192,529 14548 mRNA_6181: 14549 - K13126 PABPC polyadenylate-binding protein, at 192,986 to 195,356 14549 mRNA_6182: 14550 - K06665 SSN6 glucose repression mediator protein, at 196,153 to 199,901 14550 mRNA_6183: 14551 - Hypothetical Protein, at 200,560 to 201,520 14551
Group Condition mRNA_6179 mRNA_6180 mRNA_6181 mRNA_6182 mRNA_6183
14547 14548 14549 14550 14551
facs on lipid accumulation Sorted Low BODIPY Gate -1.3 -1.7 N.D. -2.9 -0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.9 -1.2 N.D. -0.5 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 -0.8 N.D. -1.3 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.8 +0.2 N.D. -0.8 +0.0
auxotrophy YNB Glucose -0.3 -0.3 N.D. -0.6 -0.1
growth on ricinoleic acid YNB Glucose -0.3 -0.7 N.D. -0.4 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 -0.7 N.D. -0.5 +0.1
growth on oleic acid YNB Glucose -0.3 -0.5 N.D. -0.3 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.9 +0.1 N.D. -2.0 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.2 N.D. -0.4 -0.2
growth on ypd YPD -1.1 -0.4 N.D. +0.9 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.6 -1.2 N.D. +1.0 +0.3
auxotrophy Dropout Complete +0.0 -0.3 N.D. -0.2 -0.0
auxotrophy YNB Glucose 75mM Methionine +0.0 -0.1 N.D. -0.1 -0.1
growth on oleic acid YNB Oleic Acid -0.1 -0.1 N.D. -0.0 +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.5 -0.1 N.D. +0.3 -0.0
ypd buoyancy 40 hrs Low Buoyancy -0.4 +0.0 N.D. +0.1 +0.2
ypd buoyancy 40 hrs High Buoyancy -0.7 +0.7 N.D. +0.1 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 +0.1 N.D. +0.4 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.7 -0.2 N.D. +0.0 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 +0.3 N.D. +1.8 -0.0
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