Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14562 and 14563 are separated by 177 nucleotides14563 and 14564 are separated by 452 nucleotides14564 and 14565 are separated by 134 nucleotides14565 and 14566 overlap by 2 nucleotides mRNA_6194: 14562 - SUPERFAMILY--SSF56112, at 222,804 to 223,699 14562 mRNA_6195: 14563 - SUPERFAMILY--SSF52047, at 223,877 to 225,110 14563 mRNA_6196: 14564 - Hypothetical Protein, at 225,563 to 227,023 14564 mRNA_6197: 14565 - K01885 EARS, gltX glutamyl-tRNA synthetase, at 227,158 to 229,573 14565 mRNA_6198: 14566 - BLAST tyrosine kinase domain protein [Rhizoctonia solani AG..., at 229,572 to 230,681 14566
Group Condition mRNA_6194 mRNA_6195 mRNA_6196 mRNA_6197 mRNA_6198
14562 14563 14564 14565 14566
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 -0.1 -1.8 N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.6 -0.9 N.D. +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.6 -0.2 N.D. -1.3
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.1 -0.2 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 +0.4 -0.4 N.D. -0.7
growth on oleic acid YNB Glucose +0.3 -0.2 -0.4 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.5 -0.2 N.D. -0.4
growth on oleic acid YNB Oleic Acid +0.2 -0.1 +0.1 N.D. -0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 +0.4 +0.8 N.D. -0.5
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 +1.2 -0.4 N.D. -0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.6 +1.0 -0.5 N.D. -0.6
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 +0.5 -0.3 N.D. -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.3 +0.5 N.D. -0.0
growth on ypd YPD +0.1 +0.8 -0.1 N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.3 +0.6 N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine +0.2 +0.9 -0.3 N.D. +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.7 +0.5 -0.1 N.D. +0.1
auxotrophy YNB Glucose +0.6 +1.0 -0.6 N.D. +0.2
growth on ricinoleic acid YNB Glucose +0.5 +1.2 -0.5 N.D. +0.1
auxotrophy Dropout Complete +0.2 +0.6 -0.1 N.D. +0.8
auxotrophy YNB Glucose 75mM Arginine +0.4 +1.0 +0.1 N.D. +0.2
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