Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14574 and 14575 are separated by 562 nucleotides14575 and 14576 are separated by 683 nucleotides14576 and 14577 are separated by 1361 nucleotides14577 and 14578 are separated by 1280 nucleotides mRNA_6206: 14574 - K10524 NRK1_2 nicotinamide/nicotinate riboside kinase, at 246,520 to 247,686 14574 mRNA_6207: 14575 - KOG2711 Glycerol-3-phosphate dehydrogenase/dihydroxyacetone 3-phosphate reductase, at 248,249 to 248,882 14575 mRNA_6208: 14576 - K00006 GPD1 glycerol-3-phosphate dehydrogenase (NAD+), at 249,566 to 251,082 14576 mRNA_6209: 14577 - K03006 RPB1, POLR2A DNA-directed RNA polymerase II subunit RPB1, at 252,444 to 258,337 14577 mRNA_6210: 14578 - HMMPfam-DNA replication and checkpoint protein-PF11719, at 259,618 to 261,776 14578
Group Condition mRNA_6206 mRNA_6207 mRNA_6208 mRNA_6209 mRNA_6210
14574 14575 14576 14577 14578
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 +0.0 -2.4 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 -0.7 -0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -0.8 +0.5 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.0 -0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.5 -0.5 +0.7 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 -0.5 +0.4 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.4 +0.1 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.3 +0.3 N.D. N.D.
auxotrophy YNB Glucose -0.1 -0.2 +0.3 N.D. N.D.
growth on ricinoleic acid YNB Glucose -0.3 +0.1 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 -0.6 +0.8 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.3 +0.6 N.D. N.D.
growth on ypd YPD +0.1 -0.1 +0.2 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.4 -0.0 -0.1 N.D. N.D.
growth on oleic acid YNB Oleic Acid +0.1 -0.0 +0.2 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 +0.0 +0.6 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.2 -0.0 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine +0.0 -0.1 +0.4 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.3 +0.6 -0.5 N.D. N.D.
growth on oleic acid YNB Glucose +0.3 -0.2 +0.3 N.D. N.D.
auxotrophy Dropout Complete +0.0 -0.0 +0.4 N.D. N.D.
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