Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14577 and 14578 are separated by 1280 nucleotides14578 and 14579 are separated by 94 nucleotides14579 and 14580 are separated by 1241 nucleotides14580 and 14581 are separated by 115 nucleotides mRNA_6209: 14577 - K03006 RPB1, POLR2A DNA-directed RNA polymerase II subunit RPB1, at 252,444 to 258,337 14577 mRNA_6210: 14578 - HMMPfam-DNA replication and checkpoint protein-PF11719, at 259,618 to 261,776 14578 mRNA_6211: 14579 - Hypothetical Protein, at 261,871 to 262,723 14579 mRNA_6212: 14580 - SMART-Domain with 2 conserved Trp (W) residues-SM00456,SUPERFAMILY--SSF51045, at 263,965 to 264,991 14580 mRNA_6213: 14581 - KOG2318 Uncharacterized conserved protein, at 265,107 to 268,005 14581
Group Condition mRNA_6209 mRNA_6210 mRNA_6211 mRNA_6212 mRNA_6213
14577 14578 14579 14580 14581
auxotrophy Dropout Complete N.D. N.D. -0.2 -1.6 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.4 -1.8 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.4 -1.5 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -1.3 +0.3 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.3 -0.6 N.D.
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.1 -1.0 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -2.2 +1.4 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.4 -1.0 N.D.
growth on ypd YPD N.D. N.D. +0.2 -0.6 N.D.
auxotrophy YNB Glucose N.D. N.D. -0.0 -0.4 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.5 -0.5 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.0 +0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.0 +0.4 N.D.
growth on oleic acid YNB Glucose N.D. N.D. +0.1 +0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.9 +1.7 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.8 +1.9 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.2 +1.4 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.7 +2.1 N.D.
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.5 +1.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.0 +1.8 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.2 +2.1 N.D.
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