Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14578 and 14579 are separated by 94 nucleotides14579 and 14580 are separated by 1241 nucleotides14580 and 14581 are separated by 115 nucleotides14581 and 14582 are separated by 718 nucleotides mRNA_6210: 14578 - HMMPfam-DNA replication and checkpoint protein-PF11719, at 259,618 to 261,776 14578 mRNA_6211: 14579 - Hypothetical Protein, at 261,871 to 262,723 14579 mRNA_6212: 14580 - SMART-Domain with 2 conserved Trp (W) residues-SM00456,SUPERFAMILY--SSF51045, at 263,965 to 264,991 14580 mRNA_6213: 14581 - KOG2318 Uncharacterized conserved protein, at 265,107 to 268,005 14581 mRNA_6214: 14582 - K11684 BDF1 bromodomain-containing factor 1, at 268,724 to 272,351 14582
Group Condition mRNA_6210 mRNA_6211 mRNA_6212 mRNA_6213 mRNA_6214
14578 14579 14580 14581 14582
auxotrophy Dropout Complete N.D. -0.2 -1.6 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. +0.4 -1.8 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.4 -1.5 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -1.3 +0.3 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. -0.3 -0.6 N.D. N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.1 -1.0 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -2.2 +1.4 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. +0.4 -1.0 N.D. N.D.
growth on ypd YPD N.D. +0.2 -0.6 N.D. N.D.
auxotrophy YNB Glucose N.D. -0.0 -0.4 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.5 -0.5 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.0 +0.2 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.0 +0.4 N.D. N.D.
growth on oleic acid YNB Glucose N.D. +0.1 +0.4 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.9 +1.7 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.8 +1.9 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 +1.4 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.7 +2.1 N.D. N.D.
growth on oleic acid YNB Oleic Acid N.D. +0.5 +1.1 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.0 +1.8 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.2 +2.1 N.D. N.D.
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