Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14584 and 14585 are separated by 329 nucleotides14585 and 14586 are separated by 705 nucleotides14586 and 14587 are separated by 435 nucleotides14587 and 14588 are separated by 2453 nucleotides mRNA_6216: 14584 - K03120 TBP, tbp transcription initiation factor TFIID TATA-box-binding protein, at 274,096 to 275,401 14584 mRNA_6217: 14585 - K17362 ACOT13 acyl-coenzyme A thioesterase 13, at 275,731 to 276,667 14585 mRNA_6218: 14586 - K03687 GRPE molecular chaperone GrpE, at 277,373 to 277,948 14586 mRNA_6219: 14587 - HMMPfam-Sec20-PF03908, at 278,384 to 280,109 14587 mRNA_6220: 14588 - Hypothetical Protein, at 282,563 to 283,196 14588
Group Condition mRNA_6216 mRNA_6217 mRNA_6218 mRNA_6219 mRNA_6220
14584 14585 14586 14587 14588
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.3 N.D. -0.7 -0.6
growth on ricinoleic acid YNB Glucose N.D. -0.3 N.D. -0.7 -0.5
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.3 N.D. -0.5 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.9 N.D. -0.2 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.6 N.D. -0.2 -1.4
ypd buoyancy 40 hrs High Buoyancy N.D. -0.2 N.D. +0.1 -0.6
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.3 N.D. -0.2 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.1 N.D. -0.8 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.2 N.D. -0.8 +0.7
auxotrophy YNB Glucose N.D. -0.1 N.D. +0.5 -0.6
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.2 N.D. +0.2 -0.2
auxotrophy Dropout Complete N.D. -0.1 N.D. +0.2 -0.2
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 N.D. +0.5 -0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.3 N.D. +0.1 +0.2
growth on oleic acid YNB Glucose N.D. -0.4 N.D. +0.1 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.4 N.D. -0.5 +0.9
auxotrophy YNB Glucose 75mM Methionine N.D. +0.0 N.D. +0.7 -0.7
growth on ypd YPD N.D. +0.2 N.D. +0.0 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.2 N.D. -0.8 +1.4
growth on oleic acid YNB Oleic Acid N.D. -0.0 N.D. -0.1 +0.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 N.D. +0.0 +1.5
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