Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14597 and 14598 are separated by 830 nucleotides14598 and 14599 are separated by 329 nucleotides14599 and 14600 are separated by 231 nucleotides14600 and 14601 are separated by 516 nucleotides mRNA_6229: 14597 - K01895 ACSS, acs acetyl-CoA synthetase, at 302,429 to 305,318 14597 mRNA_6230: 14598 - K10688 UBE2W, UBC16 ubiquitin-conjugating enzyme E2 W, at 306,149 to 307,289 14598 mRNA_6231: 14599 - KOG2358 NifU-like domain-containing proteins, at 307,619 to 308,722 14599 mRNA_6232: 14600 - K17450 ACO2 homoaconitase, at 308,954 to 311,929 14600 mRNA_6233: 14601 - K16945 CDC11 cell division control protein 11, at 312,446 to 314,280 14601
Group Condition mRNA_6229 mRNA_6230 mRNA_6231 mRNA_6232 mRNA_6233
14597 14598 14599 14600 14601
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.9 +0.6 -4.3 -0.7 -0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 +0.2 -5.0 -0.5 -0.4
growth on oleic acid YNB Glucose +0.2 -0.3 -1.2 -1.6 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.7 +0.6 -2.1 -0.3 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.1 -1.0 -1.3 -0.5
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.1 -0.7 -1.4 -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.6 -0.3 -0.5 -0.3 -0.3
auxotrophy YNB Glucose +0.0 +0.3 -0.6 -1.3 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.0 -0.2 -1.0 -1.8 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 -0.5 -1.1 -0.1 +0.0
facs on lipid accumulation Sorted High BODIPY Gate -1.4 +0.9 -0.1 -1.0 -0.2
growth on ricinoleic acid YNB Glucose +0.0 +0.1 -0.8 -1.2 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid +1.0 +0.3 -0.5 -1.5 +0.2
growth on oleic acid YNB Oleic Acid +0.2 -0.3 -0.1 -0.2 +0.0
auxotrophy Dropout Complete -0.1 +0.3 -0.0 -0.2 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.6 +0.2 -0.6 -0.9 +0.8
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.2 -0.1 +0.4 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.0 +0.2 -0.2 +0.4 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.2 -0.0 +0.3 +0.1
growth on ypd YPD +0.0 +0.3 +0.2 +0.5 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 +0.1 +1.0 +0.5 +0.1
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