Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14620 and 14621 are separated by 1016 nucleotides14621 and 14622 are separated by 401 nucleotides14622 and 14623 are separated by 174 nucleotides14623 and 14624 are separated by 547 nucleotides mRNA_6252: 14620 - K10845 TTDA, GTF2H5, TFB5 TFIIH basal transcription factor complex TTD-A subunit, at 358,832 to 359,230 14620 mRNA_6253: 14621 - HMMPfam-FR47-like protein-PF08445,ProSiteProfiles-Gcn5-related N-acetyltransferase (GNAT) domain profile.-PS51186,SUPERFAMILY--SSF55729, at 360,247 to 361,370 14621 mRNA_6254: 14622 - K20477 RGP1 RAB6A-GEF complex partner protein 2, at 361,772 to 365,441 14622 mRNA_6255: 14623 - K01452 E3.5.1.41 chitin deacetylase, at 365,616 to 367,386 14623 mRNA_6256: 14624 - Hypothetical Protein, at 367,934 to 368,444 14624
Group Condition mRNA_6252 mRNA_6253 mRNA_6254 mRNA_6255 mRNA_6256
14620 14621 14622 14623 14624
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 -3.2 -1.1 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 -0.8 -0.5 -0.8
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.4 -0.6 -0.4 -1.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.2 -2.3 -0.2 +0.4
auxotrophy YNB Glucose 75mM Methionine N.D. -0.0 -0.8 -0.3 -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. -0.2 -0.4 -0.1 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.1 -1.1 +0.3 -0.1
growth on oleic acid YNB Oleic Acid N.D. -0.4 -0.3 -0.2 +0.0
growth on ricinoleic acid YNB Glucose N.D. +0.4 -0.2 -0.6 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.5 +0.0 -0.5 +0.3
growth on oleic acid YNB Glucose N.D. -0.5 -0.2 +0.1 +0.1
growth on ypd YPD N.D. +0.2 +0.1 -0.5 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.2 -0.3 -0.2 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 -0.6 +0.2 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.4 -1.7 +0.7 +0.5
auxotrophy YNB Glucose N.D. +0.0 -0.5 +0.2 +0.2
auxotrophy Dropout Complete N.D. -0.2 +0.0 +0.0 +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 -0.1 -0.1 +0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.2 +0.1 +0.0 +0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 +0.5 +0.2 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.0 +0.8 +0.1 +0.2
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