Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14621 and 14622 are separated by 401 nucleotides14622 and 14623 are separated by 174 nucleotides14623 and 14624 are separated by 547 nucleotides14624 and 14625 are separated by 257 nucleotides mRNA_6253: 14621 - HMMPfam-FR47-like protein-PF08445,ProSiteProfiles-Gcn5-related N-acetyltransferase (GNAT) domain profile.-PS51186,SUPERFAMILY--SSF55729, at 360,247 to 361,370 14621 mRNA_6254: 14622 - K20477 RGP1 RAB6A-GEF complex partner protein 2, at 361,772 to 365,441 14622 mRNA_6255: 14623 - K01452 E3.5.1.41 chitin deacetylase, at 365,616 to 367,386 14623 mRNA_6256: 14624 - Hypothetical Protein, at 367,934 to 368,444 14624 mRNA_6257: 14625 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 368,702 to 371,060 14625
Group Condition mRNA_6253 mRNA_6254 mRNA_6255 mRNA_6256 mRNA_6257
14621 14622 14623 14624 14625
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -3.2 -1.1 +0.3 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.8 -0.5 -0.8 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -2.3 -0.2 +0.4 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 -0.6 -0.4 -1.2 +0.0
auxotrophy YNB Glucose 75mM Methionine -0.0 -0.8 -0.3 -0.0 -0.2
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.4 -0.1 -0.3 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 -1.7 +0.7 +0.5 -1.1
growth on oleic acid YNB Oleic Acid -0.4 -0.3 -0.2 +0.0 +0.1
growth on oleic acid YNB Glucose -0.5 -0.2 +0.1 +0.1 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -1.1 +0.3 -0.1 +0.4
growth on ypd YPD +0.2 +0.1 -0.5 -0.3 +0.0
growth on ricinoleic acid YNB Glucose +0.4 -0.2 -0.6 -0.3 +0.4
auxotrophy YNB Glucose +0.0 -0.5 +0.2 +0.2 -0.1
auxotrophy Dropout Complete -0.2 +0.0 +0.0 +0.1 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.3 -0.2 +0.1 +0.5
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 +0.0 -0.5 +0.3 +0.8
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.6 +0.2 +0.0 +0.6
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.1 -0.1 +0.2 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.1 +0.0 +0.4 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.5 +0.2 +0.3 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 +0.8 +0.1 +0.2 +0.6
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