Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14622 and 14623 are separated by 174 nucleotides14623 and 14624 are separated by 547 nucleotides14624 and 14625 are separated by 257 nucleotides14625 and 14626 are separated by 842 nucleotides mRNA_6254: 14622 - K20477 RGP1 RAB6A-GEF complex partner protein 2, at 361,772 to 365,441 14622 mRNA_6255: 14623 - K01452 E3.5.1.41 chitin deacetylase, at 365,616 to 367,386 14623 mRNA_6256: 14624 - Hypothetical Protein, at 367,934 to 368,444 14624 mRNA_6257: 14625 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 368,702 to 371,060 14625 mRNA_6258: 14626 - K01687 ilvD dihydroxy-acid dehydratase, at 371,903 to 374,480 14626
Group Condition mRNA_6254 mRNA_6255 mRNA_6256 mRNA_6257 mRNA_6258
14622 14623 14624 14625 14626
facs on lipid accumulation Sorted High BODIPY Gate -3.2 -1.1 +0.3 -0.2 +0.5
facs on lipid accumulation Sorted Low BODIPY Gate -1.7 +0.7 +0.5 -1.1 -0.6
growth on ricinoleic acid YNB Ricinoleic Acid -0.6 -0.4 -1.2 +0.0 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.8 -0.5 -0.8 -0.1 +0.2
facs on lipid accumulation Sorted No BODIPY Gate -2.3 -0.2 +0.4 -0.1 +0.3
auxotrophy YNB Glucose 75mM Methionine -0.8 -0.3 -0.0 -0.2 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.4 -0.1 -0.3 -0.2 +0.0
growth on oleic acid YNB Oleic Acid -0.3 -0.2 +0.0 +0.1 -0.4
growth on ypd YPD +0.1 -0.5 -0.3 +0.0 -0.0
growth on oleic acid YNB Glucose -0.2 +0.1 +0.1 -0.3 -0.2
auxotrophy YNB Glucose -0.5 +0.2 +0.2 -0.1 -0.2
growth on ricinoleic acid YNB Glucose -0.2 -0.6 -0.3 +0.4 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.1 +0.3 -0.1 +0.4 +0.3
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.1 +0.2 +0.1 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -0.2 +0.1 +0.5 -0.0
auxotrophy Dropout Complete +0.0 +0.0 +0.1 -0.1 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.6 +0.2 +0.0 +0.6 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 -0.5 +0.3 +0.8 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.0 +0.4 +0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.5 +0.2 +0.3 +0.3 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.8 +0.1 +0.2 +0.6 +0.2
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