Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14623 and 14624 are separated by 547 nucleotides14624 and 14625 are separated by 257 nucleotides14625 and 14626 are separated by 842 nucleotides14626 and 14627 are separated by 517 nucleotides mRNA_6255: 14623 - K01452 E3.5.1.41 chitin deacetylase, at 365,616 to 367,386 14623 mRNA_6256: 14624 - Hypothetical Protein, at 367,934 to 368,444 14624 mRNA_6257: 14625 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 368,702 to 371,060 14625 mRNA_6258: 14626 - K01687 ilvD dihydroxy-acid dehydratase, at 371,903 to 374,480 14626 mRNA_6259: 14627 - HMMPfam-Polysaccharide deacetylase-PF01522,ProSiteProfiles-Prokaryotic membrane lipoprotein lipid attachment site profile.-PS51257,ProSiteProfiles-NodB homology domain profile.-PS51677,SUPERFAMILY--SSF88713, at 374,998 to 377,098 14627
Group Condition mRNA_6255 mRNA_6256 mRNA_6257 mRNA_6258 mRNA_6259
14623 14624 14625 14626 14627
facs on lipid accumulation Sorted Low BODIPY Gate +0.7 +0.5 -1.1 -0.6 -1.9
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 -0.8 -0.1 +0.2 -0.9
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 -1.2 +0.0 +0.2 -0.4
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.3 -0.2 +0.0 -0.3
growth on ypd YPD -0.5 -0.3 +0.0 -0.0 -0.0
facs on lipid accumulation Sorted High BODIPY Gate -1.1 +0.3 -0.2 +0.5 -0.2
auxotrophy YNB Glucose 75mM Methionine -0.3 -0.0 -0.2 -0.1 -0.1
growth on ricinoleic acid YNB Glucose -0.6 -0.3 +0.4 +0.2 -0.4
growth on oleic acid YNB Glucose +0.1 +0.1 -0.3 -0.2 -0.4
auxotrophy YNB Glucose +0.2 +0.2 -0.1 -0.2 -0.3
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.2 +0.1 -0.3 -0.3
growth on oleic acid YNB Oleic Acid -0.2 +0.0 +0.1 -0.4 +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.2 +0.4 -0.1 +0.3 -0.7
auxotrophy Dropout Complete +0.0 +0.1 -0.1 +0.2 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 +0.1 +0.5 -0.0 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.4 +0.1 -0.1 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 +0.3 +0.8 -0.2 -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.2 +0.3 +0.3 -0.1 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 -0.1 +0.4 +0.3 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.0 +0.6 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.2 +0.6 +0.2 +0.4
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