Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14633 and 14634 are separated by 1543 nucleotides14634 and 14635 are separated by 219 nucleotides14635 and 14636 are separated by 167 nucleotides14636 and 14637 are separated by 213 nucleotides mRNA_6265: 14633 - K07897 RAB7A Ras-related protein Rab-7A, at 393,970 to 395,012 14633 mRNA_6266: 14634 - Hypothetical Protein, at 396,556 to 399,256 14634 mRNA_6267: 14635 - KOG1721 FOG Zn-finger, at 399,476 to 403,885 14635 mRNA_6268: 14636 - HMMPfam-Survival motor neuron protein (SMN)-PF06003, at 404,053 to 405,342 14636 mRNA_6269: 14637 - KOG1336 Monodehydroascorbate/ferredoxin reductase, at 405,556 to 407,019 14637
Group Condition mRNA_6265 mRNA_6266 mRNA_6267 mRNA_6268 mRNA_6269
14633 14634 14635 14636 14637
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.3 -0.9 -0.4
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.5 +0.0 -0.5
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -1.0 +0.5 +0.1
growth on ypd YPD N.D. N.D. +0.1 +0.2 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.3 +0.2 +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.5 +0.0 +0.4
growth on oleic acid YNB Glucose N.D. N.D. +0.6 +0.2 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.2 +0.3 +0.6
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.4 +0.9 +0.6
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.6 +0.5 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +1.0 -0.8 +1.0
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.1 +0.7 +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.6 +0.6 +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.3 +1.8 -0.5
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.2 +1.0 +0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.7 +1.1 +0.2
auxotrophy Dropout Complete N.D. N.D. +0.5 +1.6 -0.1
auxotrophy YNB Glucose N.D. N.D. +0.3 +2.4 -0.3
growth on oleic acid YNB Oleic Acid N.D. N.D. +2.1 +0.1 +0.6
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.3 +2.6 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +1.6 +0.7 +0.6
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