Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14639 and 14640 are separated by 1769 nucleotides14640 and 14641 are separated by 572 nucleotides14641 and 14642 are separated by 1581 nucleotides14642 and 14643 are separated by 360 nucleotides mRNA_6271: 14639 - Hypothetical Protein, at 410,534 to 411,923 14639 mRNA_6272: 14640 - KOG4679 Uncharacterized protein PSP1 (suppressor of DNA polymerase alpha mutations in yeast), at 413,693 to 414,563 14640 mRNA_6273: 14641 - HMMPfam-Chitin synthase III catalytic subunit-PF12271, at 415,136 to 416,506 14641 mRNA_6274: 14642 - Hypothetical Protein, at 418,088 to 419,666 14642 mRNA_6275: 14643 - BLAST secreted protein [Melampsora larici-populina 98AG31], at 420,027 to 422,108 14643
Group Condition mRNA_6271 mRNA_6272 mRNA_6273 mRNA_6274 mRNA_6275
14639 14640 14641 14642 14643
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.1 N.D. -0.4 -0.6
auxotrophy Dropout Complete +0.0 -0.4 N.D. +0.4 -0.8
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.3 N.D. +0.2 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.6 N.D. +0.4 -0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.2 +0.0 N.D. +0.0 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 +0.5 N.D. +0.1 -0.8
auxotrophy YNB Glucose -0.2 -0.0 N.D. +0.2 -0.3
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.3 N.D. +0.5 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 +0.7 N.D. +0.0 -0.4
growth on oleic acid YNB Glucose -0.1 -0.2 N.D. +0.4 +0.3
growth on ypd YPD +0.2 +0.3 N.D. -0.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.0 +0.1 N.D. +0.2 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.2 N.D. -0.2 +0.4
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.4 N.D. -0.2 +0.4
growth on ricinoleic acid YNB Glucose -0.0 +0.5 N.D. +0.3 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.7 N.D. +0.3 -0.3
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.8 N.D. -0.3 +0.5
growth on oleic acid YNB Oleic Acid -0.0 +0.3 N.D. +0.3 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.6 N.D. +0.5 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.9 N.D. +0.3 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 +1.9 N.D. +0.3 -0.0
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