Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14640 and 14641 are separated by 572 nucleotides14641 and 14642 are separated by 1581 nucleotides14642 and 14643 are separated by 360 nucleotides14643 and 14644 are separated by 793 nucleotides mRNA_6272: 14640 - KOG4679 Uncharacterized protein PSP1 (suppressor of DNA polymerase alpha mutations in yeast), at 413,693 to 414,563 14640 mRNA_6273: 14641 - HMMPfam-Chitin synthase III catalytic subunit-PF12271, at 415,136 to 416,506 14641 mRNA_6274: 14642 - Hypothetical Protein, at 418,088 to 419,666 14642 mRNA_6275: 14643 - BLAST secreted protein [Melampsora larici-populina 98AG31], at 420,027 to 422,108 14643 mRNA_6276: 14644 - K12615 EDC3 enhancer of mRNA-decapping protein 3, at 422,902 to 424,921 14644
Group Condition mRNA_6272 mRNA_6273 mRNA_6274 mRNA_6275 mRNA_6276
14640 14641 14642 14643 14644
auxotrophy Dropout Complete -0.4 N.D. +0.4 -0.8 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 N.D. -0.4 -0.6 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 N.D. +0.1 -0.8 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.6 N.D. +0.4 -0.3 -0.1
auxotrophy YNB Glucose 75mM Methionine -0.3 N.D. +0.2 -0.4 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.7 N.D. +0.0 -0.4 -0.3
auxotrophy YNB Glucose -0.0 N.D. +0.2 -0.3 +0.0
auxotrophy YNB Glucose 75mM Arginine -0.3 N.D. +0.5 -0.1 -0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.0 N.D. +0.0 -0.3 +0.3
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. -0.2 +0.4 -0.2
growth on oleic acid YNB Glucose -0.2 N.D. +0.4 +0.3 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 N.D. +0.5 -0.2 -0.6
growth on ypd YPD +0.3 N.D. -0.1 -0.1 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.7 N.D. +0.3 -0.3 -0.4
ypd buoyancy 40 hrs Median Buoyancy +0.4 N.D. -0.2 +0.4 +0.0
growth on ricinoleic acid YNB Glucose +0.5 N.D. +0.3 -0.2 -0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. +0.2 +0.1 +0.2
growth on oleic acid YNB Oleic Acid +0.3 N.D. +0.3 +0.4 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.8 N.D. -0.3 +0.5 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.9 N.D. +0.3 -0.0 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid +1.9 N.D. +0.3 -0.0 +0.2
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