Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14646 and 14647 are separated by 200 nucleotides14647 and 14648 are separated by 398 nucleotides14648 and 14649 are separated by 301 nucleotides14649 and 14650 are separated by 289 nucleotides mRNA_6278: 14646 - K17080 PHB1 prohibitin 1, at 426,858 to 428,286 14646 mRNA_6279: 14647 - K13110 MFAP1 microfibrillar-associated protein 1, at 428,487 to 430,631 14647 mRNA_6280: 14648 - K14050 RABGGTA geranylgeranyl transferase type-2 subunit alpha, at 431,030 to 432,502 14648 mRNA_6281: 14649 - HMMPfam-Stress responsive A/B Barrel Domain-PF07876,ProSiteProfiles-Stress-response A/B barrel domain profile.-PS51502,SMART-Stress responsive A/B Barrel Domain-SM00886,SUPERFAMILY--SSF54909, at 432,804 to 433,205 14649 mRNA_6282: 14650 - HMMPfam-SET domain-PF00856,HMMPfam-TPR repeat-PF13414,ProSiteProfiles-TPR repeat profile.-PS50005,ProSiteProfiles-SET domain profile.-PS50280,ProSiteProfiles-TPR repeat region circular profile.-PS50293,SMART-Tetratricopeptide repeats-SM00028,SMART-SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain-SM00317,SUPERFAMILY--SSF48452,SUPERFAMILY--SSF82199, at 433,495 to 438,419 14650
Group Condition mRNA_6278 mRNA_6279 mRNA_6280 mRNA_6281 mRNA_6282
14646 14647 14648 14649 14650
ypd buoyancy 40 hrs Low Buoyancy -2.7 N.D. N.D. +0.6 +0.3
facs on lipid accumulation Sorted High BODIPY Gate -1.2 N.D. N.D. -0.1 -0.2
growth on oleic acid YNB Glucose -0.4 N.D. N.D. -0.7 -0.1
growth on oleic acid YNB Oleic Acid -0.6 N.D. N.D. -0.4 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -1.6 N.D. N.D. +0.6 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.2 N.D. N.D. -0.3 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. N.D. -0.3 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -1.0 N.D. N.D. +0.6 +0.2
auxotrophy Dropout Complete -0.5 N.D. N.D. +0.7 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.6 N.D. N.D. -0.6 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. N.D. -0.1 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.5 N.D. N.D. +0.3 +0.2
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. N.D. +0.1 +0.3
growth on ricinoleic acid YNB Glucose -0.5 N.D. N.D. +0.5 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.5 N.D. N.D. -0.3 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. N.D. -0.2 +0.4
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. N.D. +0.8 -0.1
growth on ypd YPD +1.0 N.D. N.D. -0.2 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 N.D. N.D. +0.5 +0.3
auxotrophy YNB Glucose +0.9 N.D. N.D. +0.6 -0.0
auxotrophy YNB Glucose 75mM Methionine +0.5 N.D. N.D. +1.3 -0.1
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