Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14649 and 14650 are separated by 289 nucleotides14650 and 14651 are separated by 330 nucleotides14651 and 14652 are separated by 141 nucleotides14652 and 14653 are separated by 185 nucleotides mRNA_6281: 14649 - HMMPfam-Stress responsive A/B Barrel Domain-PF07876,ProSiteProfiles-Stress-response A/B barrel domain profile.-PS51502,SMART-Stress responsive A/B Barrel Domain-SM00886,SUPERFAMILY--SSF54909, at 432,804 to 433,205 14649 mRNA_6282: 14650 - HMMPfam-SET domain-PF00856,HMMPfam-TPR repeat-PF13414,ProSiteProfiles-TPR repeat profile.-PS50005,ProSiteProfiles-SET domain profile.-PS50280,ProSiteProfiles-TPR repeat region circular profile.-PS50293,SMART-Tetratricopeptide repeats-SM00028,SMART-SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain-SM00317,SUPERFAMILY--SSF48452,SUPERFAMILY--SSF82199, at 433,495 to 438,419 14650 mRNA_6283: 14651 - HMMPfam-FHA domain-PF00498,ProSiteProfiles-Forkhead-associated (FHA) domain profile.-PS50006,SMART-Forkhead associated domain-SM00240,SUPERFAMILY--SSF49879, at 438,750 to 440,581 14651 mRNA_6284: 14652 - BLAST macrofage activating glycoprotein, putative [Rhizocto..., at 440,723 to 442,428 14652 mRNA_6285: 14653 - K05610 UCHL5, UCH37 ubiquitin carboxyl-terminal hydrolase L5, at 442,614 to 443,927 14653
Group Condition mRNA_6281 mRNA_6282 mRNA_6283 mRNA_6284 mRNA_6285
14649 14650 14651 14652 14653
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.6 -0.1 -0.2 -0.5 -0.6
growth on ypd YPD -0.2 +0.2 +0.1 -0.0 -1.4
facs on lipid accumulation Sorted No BODIPY Gate -0.3 -0.2 +0.2 +0.0 -0.6
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 +0.3 -0.4 -0.5 -0.8
growth on oleic acid YNB Glucose -0.7 -0.1 +0.2 -0.2 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 -0.1 +0.0 -0.5 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.1 +0.0 -0.4 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 +0.1 +0.2 -0.2 +0.2
auxotrophy YNB Glucose +0.6 -0.0 -0.1 -0.7 +0.5
growth on oleic acid YNB Oleic Acid -0.4 +0.2 +0.3 -0.1 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.6 +0.2 +0.1 -0.5 -0.2
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.3 -0.2 -0.2 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 +0.4 +0.0 +0.6 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.6 +0.1 +0.1 -0.1 -0.1
auxotrophy Dropout Complete +0.7 -0.3 +0.2 -0.5 +0.5
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.2 -0.3 +0.8 +0.4
auxotrophy YNB Glucose 75mM Arginine +0.8 -0.1 -0.1 -0.7 +0.8
growth on ricinoleic acid YNB Glucose +0.5 +0.2 +0.2 -0.3 +0.1
auxotrophy YNB Glucose 75mM Methionine +1.3 -0.1 -0.0 -0.6 +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.6 +0.3 +0.3 -0.1 -0.0
ypd buoyancy 40 hrs Median Buoyancy +0.3 +0.2 -0.3 +0.3 +0.6
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