Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14650 and 14651 are separated by 330 nucleotides14651 and 14652 are separated by 141 nucleotides14652 and 14653 are separated by 185 nucleotides14653 and 14654 are separated by 286 nucleotides mRNA_6282: 14650 - HMMPfam-SET domain-PF00856,HMMPfam-TPR repeat-PF13414,ProSiteProfiles-TPR repeat profile.-PS50005,ProSiteProfiles-SET domain profile.-PS50280,ProSiteProfiles-TPR repeat region circular profile.-PS50293,SMART-Tetratricopeptide repeats-SM00028,SMART-SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain-SM00317,SUPERFAMILY--SSF48452,SUPERFAMILY--SSF82199, at 433,495 to 438,419 14650 mRNA_6283: 14651 - HMMPfam-FHA domain-PF00498,ProSiteProfiles-Forkhead-associated (FHA) domain profile.-PS50006,SMART-Forkhead associated domain-SM00240,SUPERFAMILY--SSF49879, at 438,750 to 440,581 14651 mRNA_6284: 14652 - BLAST macrofage activating glycoprotein, putative [Rhizocto..., at 440,723 to 442,428 14652 mRNA_6285: 14653 - K05610 UCHL5, UCH37 ubiquitin carboxyl-terminal hydrolase L5, at 442,614 to 443,927 14653 mRNA_6286: 14654 - HMMPfam-Protein of unknown function (DUF3128)-PF11326, at 444,214 to 444,568 14654
Group Condition mRNA_6282 mRNA_6283 mRNA_6284 mRNA_6285 mRNA_6286
14650 14651 14652 14653 14654
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 -0.4 -0.5 -0.8 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.2 -0.5 -0.6 N.D.
growth on ypd YPD +0.2 +0.1 -0.0 -1.4 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.2 +0.2 +0.0 -0.6 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.0 -0.6 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.0 -0.5 +0.1 N.D.
auxotrophy YNB Glucose -0.0 -0.1 -0.7 +0.5 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.0 -0.4 -0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 +0.1 -0.5 -0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.1 -0.7 +0.8 N.D.
auxotrophy Dropout Complete -0.3 +0.2 -0.5 +0.5 N.D.
growth on oleic acid YNB Glucose -0.1 +0.2 -0.2 +0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.1 -0.1 -0.1 N.D.
growth on ricinoleic acid YNB Glucose +0.2 +0.2 -0.3 +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.3 -0.2 -0.2 +0.3 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.2 -0.2 +0.2 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.3 +0.3 -0.1 -0.0 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 +0.0 +0.6 -0.4 N.D.
growth on oleic acid YNB Oleic Acid +0.2 +0.3 -0.1 +0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -0.3 +0.8 +0.4 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.2 -0.3 +0.3 +0.6 N.D.
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