Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14659 and 14660 are separated by 176 nucleotides14660 and 14661 are separated by 406 nucleotides14661 and 14662 are separated by 627 nucleotides14662 and 14663 are separated by 629 nucleotides mRNA_6291: 14659 - K12182 HGS, HRS, VPS27 hepatocyte growth factor-regulated tyrosine kinase substrate, at 453,109 to 455,308 14659 mRNA_6292: 14660 - K15082 RAD7 DNA repair protein RAD7, at 455,485 to 457,859 14660 mRNA_6293: 14661 - K18467 VPS29 vacuolar protein sorting-associated protein 29, at 458,266 to 459,644 14661 mRNA_6294: 14662 - K00928 lysC aspartate kinase, at 460,272 to 462,518 14662 mRNA_6295: 14663 - K11593 ELF2C, AGO eukaryotic translation initiation factor 2C, at 463,148 to 466,558 14663
Group Condition mRNA_6291 mRNA_6292 mRNA_6293 mRNA_6294 mRNA_6295
14659 14660 14661 14662 14663
facs on lipid accumulation Sorted Low BODIPY Gate -2.4 +0.0 -3.8 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate -1.2 -0.2 -0.9 N.D. -0.6
facs on lipid accumulation Sorted High BODIPY Gate -0.4 -0.1 -1.0 N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.2 -1.3 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 +0.1 -0.3 N.D. -0.7
growth on ypd YPD +0.2 +0.3 -1.7 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.1 -0.4 N.D. -0.8
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 -0.1 -0.7 N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 -0.1 -1.1 N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -0.3 -0.3 N.D. -0.2
growth on ricinoleic acid YNB Glucose -0.1 -0.2 -0.1 N.D. -0.2
growth on oleic acid YNB Glucose +0.3 +0.3 -0.3 N.D. -0.6
auxotrophy YNB Glucose 75mM Arginine +0.4 +0.3 -0.3 N.D. -0.4
auxotrophy YNB Glucose +0.5 -0.0 +0.0 N.D. -0.4
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.2 +0.1 N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.0 -0.1 N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine +0.5 +0.2 +0.1 N.D. -0.3
growth on oleic acid YNB Oleic Acid +0.5 +0.3 +0.2 N.D. -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.5 +0.1 +0.2 N.D. -0.2
auxotrophy Dropout Complete +1.1 -0.1 +0.2 N.D. -0.3
ypd buoyancy 40 hrs Low Buoyancy +0.5 +0.3 -0.1 N.D. +0.4
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