Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14665 and 14666 are separated by 152 nucleotides14666 and 14667 overlap by 11 nucleotides14667 and 14668 are separated by 24 nucleotides14668 and 14669 are separated by 899 nucleotides mRNA_6297: 14665 - K17970 MDV1, FIS2 mitochondrial division protein 1, at 469,151 to 471,840 14665 mRNA_6298: 14666 - K03842 ALG1 beta-1,4-mannosyltransferase, at 471,993 to 473,912 14666 mRNA_6299: 14667 - K14574 SDO1, SBDS ribosome maturation protein SDO1, at 473,902 to 475,228 14667 mRNA_6300: 14668 - Hypothetical Protein, at 475,253 to 476,197 14668 mRNA_6301: 14669 - Hypothetical Protein, at 477,097 to 477,785 14669
Group Condition mRNA_6297 mRNA_6298 mRNA_6299 mRNA_6300 mRNA_6301
14665 14666 14667 14668 14669
facs on lipid accumulation Sorted Low BODIPY Gate -0.9 N.D. N.D. -0.7 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.7 N.D. N.D. -1.0 +0.5
ypd buoyancy 40 hrs Low Buoyancy -1.0 N.D. N.D. -0.0 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 N.D. N.D. -0.6 +0.4
growth on oleic acid YNB Glucose -0.3 N.D. N.D. +0.1 -0.0
facs on lipid accumulation Sorted High BODIPY Gate -1.0 N.D. N.D. -0.3 +1.1
growth on ricinoleic acid YNB Glucose -0.2 N.D. N.D. -0.2 +0.2
growth on ypd YPD -0.2 N.D. N.D. +0.5 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 N.D. N.D. -0.2 +0.4
ypd buoyancy 40 hrs Median Buoyancy -0.3 N.D. N.D. -0.2 +0.6
growth on oleic acid YNB Oleic Acid -0.3 N.D. N.D. +0.4 -0.0
auxotrophy YNB Glucose +0.1 N.D. N.D. -0.1 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 N.D. N.D. +0.2 +0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. N.D. +0.4 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. N.D. +0.5 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 N.D. N.D. +0.1 +0.6
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 N.D. N.D. +0.1 +0.7
auxotrophy YNB Glucose 75mM Arginine +0.3 N.D. N.D. +0.4 +0.4
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. N.D. +0.4 +0.7
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. N.D. +0.5 +1.2
auxotrophy Dropout Complete +0.8 N.D. N.D. +0.3 +1.1
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