Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14666 and 14667 overlap by 11 nucleotides14667 and 14668 are separated by 24 nucleotides14668 and 14669 are separated by 899 nucleotides14669 and 14670 are separated by 412 nucleotides mRNA_6298: 14666 - K03842 ALG1 beta-1,4-mannosyltransferase, at 471,993 to 473,912 14666 mRNA_6299: 14667 - K14574 SDO1, SBDS ribosome maturation protein SDO1, at 473,902 to 475,228 14667 mRNA_6300: 14668 - Hypothetical Protein, at 475,253 to 476,197 14668 mRNA_6301: 14669 - Hypothetical Protein, at 477,097 to 477,785 14669 mRNA_6302: 14670 - K12562 AMPH amphiphysin, at 478,198 to 480,170 14670
Group Condition mRNA_6298 mRNA_6299 mRNA_6300 mRNA_6301 mRNA_6302
14666 14667 14668 14669 14670
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -1.0 +0.5 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.6 +0.4 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.7 +0.1 +0.2
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.2 +0.2 -0.3
auxotrophy YNB Glucose N.D. N.D. -0.1 +0.2 -0.2
growth on oleic acid YNB Glucose N.D. N.D. +0.1 -0.0 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.2 +0.4 -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.2 +0.6 -0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.3 +1.1 -0.7
growth on ypd YPD N.D. N.D. +0.5 -0.4 +0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.4 -0.0 +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.5 +0.2 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.1 +0.6 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.0 +0.3 +0.4
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.4 +0.4 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.4 +0.2 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.1 +0.7 +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.4 +0.7 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.2 +0.6 +0.4
auxotrophy Dropout Complete N.D. N.D. +0.3 +1.1 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.5 +1.2 +0.0
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