Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14679 and 14680 are separated by 466 nucleotides14680 and 14681 are separated by 425 nucleotides14681 and 14682 are separated by 378 nucleotides14682 and 14683 are separated by 549 nucleotides mRNA_6311: 14679 - K03514 PAPD5_7, TRF4 non-canonical poly(A) RNA polymerase PAPD5/7, at 505,082 to 507,881 14679 mRNA_6312: 14680 - K10754 RFC1 replication factor C subunit 1, at 508,348 to 511,991 14680 mRNA_6313: 14681 - K10592 HUWE1, MULE, ARF-BP1 E3 ubiquitin-protein ligase HUWE1, at 512,417 to 524,941 14681 mRNA_6314: 14682 - K11426 SMYD SET and MYND domain-containing protein, at 525,320 to 527,665 14682 mRNA_6315: 14683 - K10609 CUL4 cullin 4, at 528,215 to 531,333 14683
Group Condition mRNA_6311 mRNA_6312 mRNA_6313 mRNA_6314 mRNA_6315
14679 14680 14681 14682 14683
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 N.D. N.D. -0.4 -0.7
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. N.D. -0.0 -1.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.9 N.D. N.D. -0.5 -1.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 N.D. N.D. -0.2 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. N.D. -0.2 -0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 N.D. N.D. -0.2 -0.2
growth on ricinoleic acid YNB Glucose +0.3 N.D. N.D. -0.0 -1.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.5 N.D. N.D. -0.2 -1.1
facs on lipid accumulation Sorted No BODIPY Gate -0.3 N.D. N.D. +0.1 -0.4
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. N.D. +0.4 -1.0
auxotrophy YNB Glucose +0.2 N.D. N.D. +0.1 -0.9
ypd buoyancy 40 hrs Low Buoyancy +0.3 N.D. N.D. -0.2 -0.7
facs on lipid accumulation Sorted High BODIPY Gate -0.2 N.D. N.D. +0.2 -0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. N.D. -0.1 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. N.D. +0.1 -0.5
ypd buoyancy 40 hrs High Buoyancy +0.3 N.D. N.D. -0.1 -0.6
auxotrophy Dropout Complete +0.3 N.D. N.D. -0.0 -0.3
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. N.D. -0.1 -0.2
growth on oleic acid YNB Glucose -0.3 N.D. N.D. +0.1 +0.2
growth on oleic acid YNB Oleic Acid +0.1 N.D. N.D. +0.1 +0.1
growth on ypd YPD -0.0 N.D. N.D. +0.4 +0.5
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