Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14699 and 14700 are separated by 268 nucleotides14700 and 14701 are separated by 534 nucleotides14701 and 14702 are separated by 292 nucleotides14702 and 14703 are separated by 489 nucleotides mRNA_6331: 14699 - Hypothetical Protein, at 562,963 to 566,521 14699 mRNA_6332: 14700 - KOG0725 Reductases with broad range of substrate specificities, at 566,790 to 568,565 14700 mRNA_6333: 14701 - K08852 ERN1 serine/threonine-protein kinase/endoribonuclease IRE1, at 569,100 to 573,400 14701 mRNA_6334: 14702 - Hypothetical Protein, at 573,693 to 575,624 14702 mRNA_6335: 14703 - BLAST chloride channel protein 3 [Pseudozyma hubeiensis..., at 576,114 to 577,521 14703
Group Condition mRNA_6331 mRNA_6332 mRNA_6333 mRNA_6334 mRNA_6335
14699 14700 14701 14702 14703
growth on ricinoleic acid YNB Ricinoleic Acid -0.5 -0.2 N.D. -0.4 -0.9
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 -0.7 N.D. -0.9 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.9 +0.5 N.D. -1.1 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 -0.2 N.D. +0.2 -0.7
growth on oleic acid YNB Glucose -0.3 +0.1 N.D. -0.7 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.0 -0.2 N.D. -0.2 -0.4
facs on lipid accumulation Sorted No BODIPY Gate +0.0 -0.4 N.D. -0.5 +0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -0.5 N.D. -0.0 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 +0.0 N.D. -0.7 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.0 N.D. -0.4 -0.1
growth on ricinoleic acid YNB Glucose -0.1 -0.3 N.D. +0.2 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 +0.2 N.D. -0.2 -0.0
auxotrophy YNB Glucose -0.1 -0.1 N.D. +0.3 -0.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 +0.3 N.D. -0.1 +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.2 -0.2 N.D. +0.4 -0.0
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.1 N.D. +0.2 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.0 -0.1 N.D. +0.4 -0.1
growth on oleic acid YNB Oleic Acid +0.3 +0.2 N.D. +0.3 -0.5
auxotrophy Dropout Complete +0.4 +0.2 N.D. +0.1 -0.3
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.0 N.D. +0.2 +0.4
growth on ypd YPD -0.2 +0.5 N.D. +0.3 +0.1
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