Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14700 and 14701 are separated by 534 nucleotides14701 and 14702 are separated by 292 nucleotides14702 and 14703 are separated by 489 nucleotides14703 and 14704 are separated by 336 nucleotides mRNA_6332: 14700 - KOG0725 Reductases with broad range of substrate specificities, at 566,790 to 568,565 14700 mRNA_6333: 14701 - K08852 ERN1 serine/threonine-protein kinase/endoribonuclease IRE1, at 569,100 to 573,400 14701 mRNA_6334: 14702 - Hypothetical Protein, at 573,693 to 575,624 14702 mRNA_6335: 14703 - BLAST chloride channel protein 3 [Pseudozyma hubeiensis..., at 576,114 to 577,521 14703 mRNA_6336: 14704 - K14773 UTP23 U3 small nucleolar RNA-associated protein 23, at 577,858 to 579,187 14704
Group Condition mRNA_6332 mRNA_6333 mRNA_6334 mRNA_6335 mRNA_6336
14700 14701 14702 14703 14704
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 N.D. -0.4 -0.9 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.7 N.D. -0.9 +0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. -0.2 -0.4 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.4 N.D. -0.5 +0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. +0.2 -0.7 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 N.D. -0.7 -0.1 N.D.
growth on oleic acid YNB Glucose +0.1 N.D. -0.7 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 N.D. -1.1 +0.0 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.5 N.D. -0.0 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 N.D. -0.4 -0.1 N.D.
growth on ricinoleic acid YNB Glucose -0.3 N.D. +0.2 -0.3 N.D.
auxotrophy YNB Glucose -0.1 N.D. +0.3 -0.5 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. -0.2 -0.0 N.D.
auxotrophy Dropout Complete +0.2 N.D. +0.1 -0.3 N.D.
growth on oleic acid YNB Oleic Acid +0.2 N.D. +0.3 -0.5 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. +0.2 -0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. +0.4 -0.0 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 N.D. -0.1 +0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. +0.4 -0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. +0.2 +0.4 N.D.
growth on ypd YPD +0.5 N.D. +0.3 +0.1 N.D.
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