Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14708 and 14709 are separated by 260 nucleotides14709 and 14710 are separated by 257 nucleotides14710 and 14711 are separated by 390 nucleotides14711 and 14712 are separated by 582 nucleotides mRNA_6340: 14708 - BLAST tryptophan--tRNA ligase [Puccinia sorghi], at 586,004 to 586,792 14708 mRNA_6341: 14709 - K01867 WARS, trpS tryptophanyl-tRNA synthetase, at 587,053 to 588,639 14709 mRNA_6342: 14710 - KOG0756 Mitochondrial tricarboxylate/dicarboxylate carrier proteins, at 588,897 to 590,459 14710 mRNA_6343: 14711 - K12621 LSM2 U6 snRNA-associated Sm-like protein LSm2, at 590,850 to 591,445 14711 mRNA_6344: 14712 - KOG3525 Subtilisin-like proprotein convertase, at 592,028 to 595,751 14712
Group Condition mRNA_6340 mRNA_6341 mRNA_6342 mRNA_6343 mRNA_6344
14708 14709 14710 14711 14712
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.7 N.D. -0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.4 N.D. -0.3
growth on oleic acid YNB Glucose N.D. N.D. -0.4 N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.3 N.D. -0.3
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.5 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.2 N.D. -0.4
growth on ypd YPD N.D. N.D. -0.5 N.D. -0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.3 N.D. -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.2 N.D. -0.2
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.4 N.D. +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.3 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.1 N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.1 N.D. -0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.3 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.2 N.D. -0.3
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.2 N.D. +0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.0 N.D. +0.0
auxotrophy YNB Glucose N.D. N.D. -0.2 N.D. +0.3
auxotrophy Dropout Complete N.D. N.D. -0.0 N.D. +0.2
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.1 N.D. +0.3
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.2 N.D. +0.5
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