Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14719 and 14720 are separated by 942 nucleotides14720 and 14721 are separated by 1042 nucleotides14721 and 14722 are separated by 234 nucleotides14722 and 14723 are separated by 529 nucleotides mRNA_6351: 14719 - K02995 RP-S8e, RPS8 small subunit ribosomal protein S8e, at 612,395 to 613,472 14719 mRNA_6352: 14720 - K02980 RP-S29e, RPS29 small subunit ribosomal protein S29e, at 614,415 to 614,996 14720 mRNA_6353: 14721 - KOG4660 Protein Mei2, essential for commitment to meiosis, and related proteins, at 616,039 to 616,629 14721 mRNA_6354: 14722 - BLAST Mei2p [Pneumocystis carinii], at 616,864 to 619,118 14722 mRNA_6355: 14723 - KOG4532 WD40-like repeat containing protein, at 619,648 to 622,272 14723
Group Condition mRNA_6351 mRNA_6352 mRNA_6353 mRNA_6354 mRNA_6355
14719 14720 14721 14722 14723
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. -0.1 -0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. -1.0 +0.4
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.5 -0.1
auxotrophy Dropout Complete N.D. N.D. N.D. -0.2 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. -0.2 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. -0.1 -0.1
growth on oleic acid YNB Glucose N.D. N.D. N.D. -0.2 -0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. +0.1 -0.3
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. +0.1 -0.2
auxotrophy YNB Glucose N.D. N.D. N.D. -0.1 -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. -0.2 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. -0.0 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. -0.3 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. -0.0 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. +0.1 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. -0.3 +0.4
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. -0.0 +0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. +0.0 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. -0.2 +0.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. +0.2 +0.1
growth on ypd YPD N.D. N.D. N.D. +1.0 -0.2
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