Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14720 and 14721 are separated by 1042 nucleotides14721 and 14722 are separated by 234 nucleotides14722 and 14723 are separated by 529 nucleotides14723 and 14724 are separated by 185 nucleotides mRNA_6352: 14720 - K02980 RP-S29e, RPS29 small subunit ribosomal protein S29e, at 614,415 to 614,996 14720 mRNA_6353: 14721 - KOG4660 Protein Mei2, essential for commitment to meiosis, and related proteins, at 616,039 to 616,629 14721 mRNA_6354: 14722 - BLAST Mei2p [Pneumocystis carinii], at 616,864 to 619,118 14722 mRNA_6355: 14723 - KOG4532 WD40-like repeat containing protein, at 619,648 to 622,272 14723 mRNA_6356: 14724 - K01477 alc, ALLC allantoicase, at 622,458 to 625,058 14724
Group Condition mRNA_6352 mRNA_6353 mRNA_6354 mRNA_6355 mRNA_6356
14720 14721 14722 14723 14724
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.2 +0.0 -4.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.1 -0.1 -3.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.2 +0.1 -2.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.1 -0.6 -0.7
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.0 -0.1 -0.8
auxotrophy Dropout Complete N.D. N.D. -0.2 -0.2 -0.4
growth on oleic acid YNB Glucose N.D. N.D. -0.2 -0.0 -0.4
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.5 -0.1 +0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.0 +0.1 -0.6
auxotrophy YNB Glucose N.D. N.D. -0.1 -0.1 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -1.0 +0.4 +0.2
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.1 -0.1 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.2 -0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.0 +0.1 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.2 +0.4 -0.4
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.1 -0.2 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.3 +0.4 -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.0 -0.1 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.1 -0.3 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.3 +0.2 +0.5
growth on ypd YPD N.D. N.D. +1.0 -0.2 +0.1
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