Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14724 and 14725 are separated by 308 nucleotides14725 and 14726 are separated by 482 nucleotides14726 and 14727 are separated by 1414 nucleotides14727 and 14728 are separated by 661 nucleotides mRNA_6356: 14724 - K01477 alc, ALLC allantoicase, at 622,458 to 625,058 14724 mRNA_6357: 14725 - HMMPfam-Ribonuclease-III-like-PF14622,SMART-Ribonuclease III family-SM00535,SUPERFAMILY--SSF69065, at 625,367 to 626,592 14725 mRNA_6358: 14726 - KOG1674 Cyclin, at 627,075 to 629,360 14726 mRNA_6359: 14727 - KOG1812 Predicted E3 ubiquitin ligase, at 630,775 to 633,081 14727 mRNA_6360: 14728 - K17790 TIM22 mitochondrial import inner membrane translocase subunit TIM22, at 633,743 to 634,504 14728
Group Condition mRNA_6356 mRNA_6357 mRNA_6358 mRNA_6359 mRNA_6360
14724 14725 14726 14727 14728
facs on lipid accumulation Sorted High BODIPY Gate -4.0 N.D. -0.7 -0.3 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -3.2 N.D. +0.1 -0.2 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -2.4 N.D. +0.2 -0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.7 N.D. -1.0 -0.0 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.8 N.D. -0.4 +0.2 N.D.
growth on oleic acid YNB Oleic Acid -0.6 N.D. -0.3 +0.1 N.D.
growth on oleic acid YNB Glucose -0.4 N.D. -0.3 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 N.D. +0.1 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. -0.2 +0.0 N.D.
growth on ricinoleic acid YNB Glucose -0.0 N.D. -0.2 +0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. -0.3 +0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.3 N.D. -0.0 +0.2 N.D.
auxotrophy Dropout Complete -0.4 N.D. +0.0 +0.3 N.D.
auxotrophy YNB Glucose -0.4 N.D. +0.1 +0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 N.D. +0.0 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. +0.2 -0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. -0.0 +0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.3 N.D. -0.1 +0.1 N.D.
growth on ypd YPD +0.1 N.D. +0.3 +0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.2 N.D. +0.3 +0.4 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 N.D. +0.1 -0.1 N.D.
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