Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14726 and 14727 are separated by 1414 nucleotides14727 and 14728 are separated by 661 nucleotides14728 and 14729 are separated by 763 nucleotides14729 and 14730 are separated by 559 nucleotides mRNA_6358: 14726 - KOG1674 Cyclin, at 627,075 to 629,360 14726 mRNA_6359: 14727 - KOG1812 Predicted E3 ubiquitin ligase, at 630,775 to 633,081 14727 mRNA_6360: 14728 - K17790 TIM22 mitochondrial import inner membrane translocase subunit TIM22, at 633,743 to 634,504 14728 mRNA_6361: 14729 - K08832 SRPK3, STK23 serine/threonine-protein kinase SRPK3, at 635,268 to 638,035 14729 mRNA_6362: 14730 - SMART-Cytochrome b-561 / ferric reductase transmembrane domain.-SM00665, at 638,595 to 641,031 14730
Group Condition mRNA_6358 mRNA_6359 mRNA_6360 mRNA_6361 mRNA_6362
14726 14727 14728 14729 14730
facs on lipid accumulation Sorted High BODIPY Gate -0.7 -0.3 N.D. N.D. -0.6
growth on ricinoleic acid YNB Ricinoleic Acid -1.0 -0.0 N.D. N.D. +0.4
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -0.2 N.D. N.D. -0.4
growth on oleic acid YNB Glucose -0.3 +0.2 N.D. N.D. -0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.1 N.D. N.D. -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.1 N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.1 N.D. N.D. -0.0
growth on ricinoleic acid YNB Glucose -0.2 +0.0 N.D. N.D. +0.2
ypd buoyancy 40 hrs Low Buoyancy -0.3 +0.3 N.D. N.D. +0.1
auxotrophy YNB Glucose 75mM Arginine -0.0 +0.2 N.D. N.D. -0.1
growth on oleic acid YNB Oleic Acid -0.3 +0.1 N.D. N.D. +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.1 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.2 N.D. N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy -0.0 +0.1 N.D. N.D. +0.1
auxotrophy Dropout Complete +0.0 +0.3 N.D. N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 +0.1 N.D. N.D. +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 +0.2 N.D. N.D. +0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 +0.0 N.D. N.D. +0.4
auxotrophy YNB Glucose 75mM Methionine +0.3 +0.4 N.D. N.D. -0.3
growth on ypd YPD +0.3 +0.1 N.D. N.D. +0.0
auxotrophy YNB Glucose +0.1 +0.3 N.D. N.D. +0.3
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