Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14728 and 14729 are separated by 763 nucleotides14729 and 14730 are separated by 559 nucleotides14730 and 14731 are separated by 66 nucleotides14731 and 14732 overlap by 61 nucleotides mRNA_6360: 14728 - K17790 TIM22 mitochondrial import inner membrane translocase subunit TIM22, at 633,743 to 634,504 14728 mRNA_6361: 14729 - K08832 SRPK3, STK23 serine/threonine-protein kinase SRPK3, at 635,268 to 638,035 14729 mRNA_6362: 14730 - SMART-Cytochrome b-561 / ferric reductase transmembrane domain.-SM00665, at 638,595 to 641,031 14730 mRNA_6363: 14731 - K14553 UTP18 U3 small nucleolar RNA-associated protein 18, at 641,098 to 643,745 14731 mRNA_6364: 14732 - BLAST putative protein [Melanopsichium pennsylvanicum 4], at 643,685 to 645,198 14732
Group Condition mRNA_6360 mRNA_6361 mRNA_6362 mRNA_6363 mRNA_6364
14728 14729 14730 14731 14732
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.4 -1.0 -0.9
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.1 +0.1 -2.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.1 +0.2 -1.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.0 -0.3 -0.7
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.6 +0.1 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.1 +0.4 -1.2
auxotrophy Dropout Complete N.D. N.D. -0.1 -0.1 -0.3
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.3 -0.5 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.4 -0.2 -0.7
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.1 -0.1 -0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.3 -0.4 +0.5
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.1 -0.7 +0.5
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.1 -0.3 +0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.2 -0.3 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.4 -0.1 -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.1 +0.4 +0.1
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.2 +0.2 +0.0
auxotrophy YNB Glucose N.D. N.D. +0.3 +0.4 -0.1
growth on oleic acid YNB Glucose N.D. N.D. -0.2 +0.5 +0.3
growth on ypd YPD N.D. N.D. +0.0 -0.0 +1.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.5 +0.5 +0.4
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