Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14739 and 14740 are separated by 314 nucleotides14740 and 14741 are separated by 527 nucleotides14741 and 14742 are separated by 378 nucleotides14742 and 14743 are separated by 290 nucleotides mRNA_6371: 14739 - HMMPfam-SET domain-PF00856,ProSiteProfiles-SET domain profile.-PS50280,SUPERFAMILY--SSF82199, at 659,092 to 660,021 14739 mRNA_6372: 14740 - BLAST similar to DNA repair protein Dds20/Mei5 [Botrytis ci..., at 660,336 to 661,808 14740 mRNA_6373: 14741 - K03013 RPB5, POLR2E DNA-directed RNA polymerases I, II, and III subunit RPABC1, at 662,336 to 663,388 14741 mRNA_6374: 14742 - K20464 OSBPL5_8, ORP5_8 oxysterol-binding protein-related protein 5/8, at 663,767 to 666,324 14742 mRNA_6375: 14743 - K01178 E3.2.1.3 glucoamylase, at 666,615 to 668,807 14743
Group Condition mRNA_6371 mRNA_6372 mRNA_6373 mRNA_6374 mRNA_6375
14739 14740 14741 14742 14743
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 N.D. N.D. -0.1 -0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.8 N.D. N.D. -0.0 +0.2
growth on oleic acid YNB Glucose -0.3 N.D. N.D. +0.1 -0.1
growth on oleic acid YNB Oleic Acid -0.4 N.D. N.D. +0.3 -0.2
auxotrophy YNB Glucose 75mM Arginine -0.3 N.D. N.D. +0.1 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. N.D. -0.1 -0.2
growth on ricinoleic acid YNB Glucose -0.1 N.D. N.D. +0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. N.D. -0.1 -0.1
auxotrophy YNB Glucose +0.2 N.D. N.D. +0.2 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. N.D. +0.4 -0.3
ypd buoyancy 40 hrs High Buoyancy +0.5 N.D. N.D. +0.1 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 N.D. N.D. +0.5 -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.4 N.D. N.D. +0.3 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 N.D. N.D. +0.8 +0.1
auxotrophy Dropout Complete +0.2 N.D. N.D. +0.2 +0.0
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. N.D. +0.6 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 N.D. N.D. +0.1 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 N.D. N.D. +0.2 +0.3
growth on ypd YPD +0.1 N.D. N.D. +0.0 +0.9
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.9 N.D. N.D. +0.1 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +1.1 N.D. N.D. +0.1 +0.3
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