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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
14740 and 14741 are separated by 527 nucleotides
14741 and 14742 are separated by 378 nucleotides
14742 and 14743 are separated by 290 nucleotides
14743 and 14744 are separated by 360 nucleotides
mRNA_6372: 14740 - BLAST similar to DNA repair protein Dds20/Mei5 [Botrytis ci..., at 660,336 to 661,808
14740
mRNA_6373: 14741 - K03013 RPB5, POLR2E DNA-directed RNA polymerases I, II, and III subunit RPABC1, at 662,336 to 663,388
14741
mRNA_6374: 14742 - K20464 OSBPL5_8, ORP5_8 oxysterol-binding protein-related protein 5/8, at 663,767 to 666,324
14742
mRNA_6375: 14743 - K01178 E3.2.1.3 glucoamylase, at 666,615 to 668,807
14743
mRNA_6376: 14744 - K14811 DBP3 ATP-dependent RNA helicase DBP3, at 669,168 to 671,272
14744
Group
Condition
mRNA
_6372
mRNA
_6373
mRNA
_6374
mRNA
_6375
mRNA
_6376
14740
14741
14742
14743
14744
auxotrophy
YNB Glucose 75mM Methionine
N.D.
N.D.
-0.1
-0.2
-0.1
growth on oleic acid
YNB Glucose
N.D.
N.D.
+0.1
-0.1
-0.3
auxotrophy
YNB Glucose
N.D.
N.D.
+0.2
-0.2
-0.3
facs on lipid accumulation
Sorted High BODIPY Gate
N.D.
N.D.
-0.0
+0.2
-0.4
ypd buoyancy
40 hrs Median Buoyancy
N.D.
N.D.
-0.1
-0.1
+0.2
auxotrophy
YNB Glucose 75mM Arginine
N.D.
N.D.
+0.1
-0.1
-0.1
auxotrophy
Dropout Complete
N.D.
N.D.
+0.2
+0.0
-0.2
buoyancy on lipid accumulation
40 hrs High Buoyancy
N.D.
N.D.
+0.1
+0.1
-0.1
facs on lipid accumulation
Sorted No BODIPY Gate
N.D.
N.D.
+0.3
+0.4
-0.6
growth on ricinoleic acid
YNB Glucose
N.D.
N.D.
+0.1
-0.1
+0.1
ypd buoyancy
40 hrs High Buoyancy
N.D.
N.D.
+0.1
-0.3
+0.4
growth on oleic acid
YNB Oleic Acid
N.D.
N.D.
+0.3
-0.2
+0.1
ypd buoyancy
40 hrs No Buoyancy Separation
N.D.
N.D.
-0.1
-0.4
+0.8
buoyancy on lipid accumulation
40 hrs Low Buoyancy
N.D.
N.D.
+0.1
+0.2
+0.1
buoyancy on lipid accumulation
40 hrs Median Buoyancy
N.D.
N.D.
+0.1
+0.3
+0.0
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
N.D.
N.D.
+0.2
+0.3
-0.0
growth on ricinoleic acid
YNB Ricinoleic Acid
N.D.
N.D.
+0.4
-0.3
+0.4
facs on lipid accumulation
Sorted Low BODIPY Gate
N.D.
N.D.
+0.8
+0.1
-0.5
ypd buoyancy
40 hrs Low Buoyancy
N.D.
N.D.
+0.6
-0.1
+0.2
growth on ricinoleic acid
YNB Methylricinoleic Acid
N.D.
N.D.
+0.5
-0.2
+0.4
growth on ypd
YPD
N.D.
N.D.
+0.0
+0.9
+0.1
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