Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14740 and 14741 are separated by 527 nucleotides14741 and 14742 are separated by 378 nucleotides14742 and 14743 are separated by 290 nucleotides14743 and 14744 are separated by 360 nucleotides mRNA_6372: 14740 - BLAST similar to DNA repair protein Dds20/Mei5 [Botrytis ci..., at 660,336 to 661,808 14740 mRNA_6373: 14741 - K03013 RPB5, POLR2E DNA-directed RNA polymerases I, II, and III subunit RPABC1, at 662,336 to 663,388 14741 mRNA_6374: 14742 - K20464 OSBPL5_8, ORP5_8 oxysterol-binding protein-related protein 5/8, at 663,767 to 666,324 14742 mRNA_6375: 14743 - K01178 E3.2.1.3 glucoamylase, at 666,615 to 668,807 14743 mRNA_6376: 14744 - K14811 DBP3 ATP-dependent RNA helicase DBP3, at 669,168 to 671,272 14744
Group Condition mRNA_6372 mRNA_6373 mRNA_6374 mRNA_6375 mRNA_6376
14740 14741 14742 14743 14744
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.1 -0.2 -0.1
growth on oleic acid YNB Glucose N.D. N.D. +0.1 -0.1 -0.3
auxotrophy YNB Glucose N.D. N.D. +0.2 -0.2 -0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.0 +0.2 -0.4
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.1 -0.1 +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.1 -0.1 -0.1
auxotrophy Dropout Complete N.D. N.D. +0.2 +0.0 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.1 +0.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.3 +0.4 -0.6
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.1 -0.1 +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.1 -0.3 +0.4
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.3 -0.2 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.1 -0.4 +0.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.1 +0.2 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.1 +0.3 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.2 +0.3 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.4 -0.3 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.8 +0.1 -0.5
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.6 -0.1 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.5 -0.2 +0.4
growth on ypd YPD N.D. N.D. +0.0 +0.9 +0.1
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