Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14780 and 14781 are separated by 198 nucleotides14781 and 14782 are separated by 768 nucleotides14782 and 14783 are separated by 1840 nucleotides14783 and 14784 are separated by 373 nucleotides mRNA_6412: 14780 - HMMPfam-F-box-like-PF12937, at 750,026 to 751,228 14780 mRNA_6413: 14781 - K08490 STX5 syntaxin 5, at 751,427 to 753,086 14781 mRNA_6414: 14782 - K15731 CTDSP carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase, at 753,855 to 754,896 14782 mRNA_6415: 14783 - Hypothetical Protein, at 756,737 to 757,520 14783 mRNA_6416: 14784 - Hypothetical Protein, at 757,894 to 758,331 14784
Group Condition mRNA_6412 mRNA_6413 mRNA_6414 mRNA_6415 mRNA_6416
14780 14781 14782 14783 14784
growth on ypd YPD -0.1 N.D. N.D. -0.8 -0.3
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. N.D. -1.1 +0.0
auxotrophy YNB Glucose 75mM Arginine -0.3 N.D. N.D. -0.8 +0.2
auxotrophy Dropout Complete +0.0 N.D. N.D. -0.9 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. N.D. -0.4 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 N.D. N.D. -0.9 +0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.1 N.D. N.D. -0.3 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 N.D. N.D. -0.5 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.7 N.D. N.D. -0.6 -0.4
growth on oleic acid YNB Oleic Acid +0.3 N.D. N.D. -0.6 +0.2
auxotrophy YNB Glucose +0.2 N.D. N.D. -0.4 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.7 N.D. N.D. -0.8 +0.1
growth on ricinoleic acid YNB Glucose -0.3 N.D. N.D. -0.1 +0.4
growth on oleic acid YNB Glucose -0.0 N.D. N.D. -0.4 +0.6
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. N.D. -0.4 +0.9
ypd buoyancy 40 hrs High Buoyancy -0.4 N.D. N.D. -0.3 +1.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 N.D. N.D. +0.2 -0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.4 N.D. N.D. +0.6 +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 N.D. N.D. +0.1 +0.3
ypd buoyancy 40 hrs Low Buoyancy -0.2 N.D. N.D. +0.0 +1.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 N.D. N.D. +1.1 +0.3
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