Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14801 and 14802 are separated by 197 nucleotides14802 and 14803 are separated by 232 nucleotides14803 and 14804 are separated by 192 nucleotides14804 and 14805 are separated by 275 nucleotides mRNA_6433: 14801 - K12180 COPS7, CSN7 COP9 signalosome complex subunit 7, at 789,986 to 791,469 14801 mRNA_6434: 14802 - K01904 4CL 4-coumarate--CoA ligase, at 791,667 to 793,929 14802 mRNA_6435: 14803 - K01930 FPGS folylpolyglutamate synthase, at 794,162 to 796,213 14803 mRNA_6436: 14804 - SMART-Domain with 2 conserved Trp (W) residues-SM00456,SUPERFAMILY--SSF51045, at 796,406 to 797,649 14804 mRNA_6437: 14805 - K07511 ECHS1 enoyl-CoA hydratase, at 797,925 to 799,246 14805
Group Condition mRNA_6433 mRNA_6434 mRNA_6435 mRNA_6436 mRNA_6437
14801 14802 14803 14804 14805
growth on ricinoleic acid YNB Ricinoleic Acid -0.9 -0.1 N.D. -0.0 -4.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 -0.1 N.D. +0.1 -4.0
growth on oleic acid YNB Oleic Acid -0.3 -0.1 N.D. -0.0 -2.9
facs on lipid accumulation Sorted High BODIPY Gate -1.1 -0.1 N.D. -0.5 +0.4
growth on ricinoleic acid YNB Glucose -0.6 -0.1 N.D. -0.4 +0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.1 N.D. -0.3 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.1 N.D. +0.0 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.1 N.D. -0.2 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 +0.3 N.D. -0.2 +0.1
growth on oleic acid YNB Glucose +0.2 -0.1 N.D. -0.1 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.6 +0.0 N.D. +0.1 +0.5
auxotrophy Dropout Complete +0.1 -0.0 N.D. +0.3 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 +0.3 N.D. +0.2 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.0 -0.0 N.D. +0.1 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.1 N.D. +0.1 -0.1
auxotrophy YNB Glucose +0.2 +0.1 N.D. -0.0 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.3 +0.0 N.D. +0.1 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.3 +0.1 N.D. +0.1 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 +0.2 N.D. +0.2 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.3 +0.2 N.D. -0.1 +0.4
growth on ypd YPD +0.3 -0.1 N.D. +0.4 +0.5
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