Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14803 and 14804 are separated by 192 nucleotides14804 and 14805 are separated by 275 nucleotides14805 and 14806 are separated by 370 nucleotides14806 and 14807 are separated by 189 nucleotides mRNA_6435: 14803 - K01930 FPGS folylpolyglutamate synthase, at 794,162 to 796,213 14803 mRNA_6436: 14804 - SMART-Domain with 2 conserved Trp (W) residues-SM00456,SUPERFAMILY--SSF51045, at 796,406 to 797,649 14804 mRNA_6437: 14805 - K07511 ECHS1 enoyl-CoA hydratase, at 797,925 to 799,246 14805 mRNA_6438: 14806 - K15456 KTI12 protein KTI12, at 799,617 to 800,834 14806 mRNA_6439: 14807 - Hypothetical Protein, at 801,024 to 801,752 14807
Group Condition mRNA_6435 mRNA_6436 mRNA_6437 mRNA_6438 mRNA_6439
14803 14804 14805 14806 14807
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.0 -4.2 +0.5 +0.7
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 -4.0 +0.2 +1.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 +0.2 -1.9 -0.4
growth on oleic acid YNB Oleic Acid N.D. -0.0 -2.9 +0.3 +0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 -0.1 -1.4 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 +0.1 -1.0 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 +0.5 -1.3 -0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.5 +0.4 -0.7 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.0 +0.1 -0.6 -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.2 +0.0 +0.0 -0.4
auxotrophy YNB Glucose N.D. -0.0 +0.1 -0.6 +0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.3 +0.3 -0.1 -0.1
growth on ypd YPD N.D. +0.4 +0.5 -0.9 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. -0.1 +0.4 -0.5 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 +0.1 +0.2 -0.3
growth on oleic acid YNB Glucose N.D. -0.1 -0.1 +0.2 +0.0
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 +0.2 -0.4 +0.3
growth on ricinoleic acid YNB Glucose N.D. -0.4 +0.3 +0.0 +0.4
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.2 -0.1 +0.6 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.2 +0.1 +0.9 -0.3
auxotrophy Dropout Complete N.D. +0.3 -0.1 +0.3 +0.3
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