Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14835 and 14836 are separated by 482 nucleotides14836 and 14837 are separated by 111 nucleotides14837 and 14838 are separated by 396 nucleotides14838 and 14839 are separated by 355 nucleotides mRNA_6467: 14835 - K20242 EVI5 ecotropic viral integration site 5 protein, at 850,077 to 853,413 14835 mRNA_6468: 14836 - K05012 CLCN3_4_5 chloride channel 3/4/5, at 853,896 to 857,931 14836 mRNA_6469: 14837 - K10625 UBR1 E3 ubiquitin-protein ligase UBR1, at 858,043 to 864,348 14837 mRNA_6470: 14838 - Hypothetical Protein, at 864,745 to 865,870 14838 mRNA_6471: 14839 - KOG0806 Carbon-nitrogen hydrolase, at 866,226 to 867,912 14839
Group Condition mRNA_6467 mRNA_6468 mRNA_6469 mRNA_6470 mRNA_6471
14835 14836 14837 14838 14839
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 N.D. -1.0 +0.1 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 N.D. -0.9 -0.1 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. -0.7 +0.1 -0.5
growth on ricinoleic acid YNB Glucose -0.3 N.D. -0.5 +0.0 -0.0
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. -0.2 -0.3 -0.1
auxotrophy YNB Glucose +0.1 N.D. -0.0 -0.4 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.2 N.D. +0.1 +0.2 -0.4
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. +0.2 -0.2 -0.2
auxotrophy YNB Glucose 75mM Methionine -0.1 N.D. +0.0 -0.2 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. -0.6 +0.4 +0.0
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. +0.3 -0.4 -0.0
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. -0.0 -0.0 -0.2
growth on oleic acid YNB Glucose -0.1 N.D. +0.1 -0.2 +0.1
auxotrophy Dropout Complete +0.0 N.D. +0.3 -0.3 -0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. -0.1 +0.1 -0.0
growth on oleic acid YNB Oleic Acid +0.1 N.D. -0.0 -0.0 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 N.D. -0.3 +0.5 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 N.D. +0.1 +0.1 -0.2
growth on ypd YPD +0.2 N.D. +0.7 -0.0 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. +0.4 +0.1 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. +0.6 +0.4 +0.3
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