Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14843 and 14844 are separated by 227 nucleotides14844 and 14845 are separated by 723 nucleotides14845 and 14846 are separated by 674 nucleotides14846 and 14847 are separated by 2469 nucleotides mRNA_6475: 14843 - KOG3442 Uncharacterized conserved protein, at 873,206 to 873,968 14843 mRNA_6476: 14844 - KOG0054 Multidrug resistance-associated protein/mitoxantrone resistance protein, ABC superfamily, at 874,196 to 881,716 14844 mRNA_6477: 14845 - HMMPfam-Catalytic LigB subunit of aromatic ring-opening dioxygenase-PF02900,SUPERFAMILY--SSF53213, at 882,440 to 883,452 14845 mRNA_6478: 14846 - K15442 TAD3, ADAT3 tRNA-specific adenosine deaminase 3, at 884,127 to 886,487 14846 mRNA_6479: 14847 - K07378 NLGN neuroligin, at 888,957 to 890,595 14847
Group Condition mRNA_6475 mRNA_6476 mRNA_6477 mRNA_6478 mRNA_6479
14843 14844 14845 14846 14847
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.2 -1.0 N.D. -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. -0.0 -1.0 N.D. +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.4 -0.6 N.D. -0.5
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.0 -0.7 N.D. -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.3 -0.8 N.D. -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.2 -0.7 N.D. -0.0
growth on oleic acid YNB Oleic Acid N.D. +0.0 -0.3 N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 -0.5 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.0 -0.4 N.D. +0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.2 +0.0 N.D. -0.2
growth on ricinoleic acid YNB Glucose N.D. +0.0 -0.4 N.D. +0.1
growth on oleic acid YNB Glucose N.D. -0.1 -0.2 N.D. +0.1
auxotrophy Dropout Complete N.D. +0.2 -0.4 N.D. +0.0
growth on ypd YPD N.D. +0.2 -0.3 N.D. -0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 -0.5 N.D. +0.3
auxotrophy YNB Glucose N.D. +0.0 -0.0 N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.0 -0.0 N.D. -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 -0.1 N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.0 -0.2 N.D. +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 +0.1 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. -0.0 +0.2 N.D. -0.1
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