Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14853 and 14854 are separated by 499 nucleotides14854 and 14855 are separated by 775 nucleotides14855 and 14856 are separated by 355 nucleotides14856 and 14857 are separated by 394 nucleotides mRNA_6485: 14853 - K00826 E2.6.1.42, ilvE branched-chain amino acid aminotransferase, at 898,441 to 899,753 14853 mRNA_6486: 14854 - K08294 MKK1_2 mitogen-activated protein kinase kinase, at 900,253 to 901,810 14854 mRNA_6487: 14855 - K11237 BEM1 bud emergence protein 1, at 902,586 to 904,816 14855 mRNA_6488: 14856 - K01655 LYS21, LYS20 homocitrate synthase, at 905,172 to 907,549 14856 mRNA_6489: 14857 - KOG0327 Translation initiation factor 4F, helicase subunit (eIF-4A) and related helicases, at 907,944 to 911,303 14857
Group Condition mRNA_6485 mRNA_6486 mRNA_6487 mRNA_6488 mRNA_6489
14853 14854 14855 14856 14857
ypd buoyancy 40 hrs No Buoyancy Separation -1.6 +0.3 -0.2 -1.0 -0.7
facs on lipid accumulation Sorted No BODIPY Gate +0.5 -0.0 +0.5 -3.9 +0.2
auxotrophy YNB Glucose 75mM Methionine +0.2 +0.0 +0.6 -4.3 +0.9
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.0 +0.0 -0.5 -3.2 +0.3
growth on oleic acid YNB Glucose -0.1 -0.0 +0.7 -3.8 +0.8
buoyancy on lipid accumulation 40 hrs Median Buoyancy +1.0 -0.1 +0.4 -3.3 -0.1
auxotrophy YNB Glucose -0.1 +0.5 +0.6 -3.9 +0.9
auxotrophy YNB Glucose 75mM Arginine +0.3 +0.6 +0.7 -4.3 +0.9
facs on lipid accumulation Sorted Low BODIPY Gate +0.9 +0.7 +0.4 -4.1 +0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.2 +0.2 +0.8 -3.1 +0.2
growth on oleic acid YNB Oleic Acid -0.1 -0.1 +0.7 -2.9 +1.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.2 +0.1 +0.5 -3.2 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +1.2 +0.2 +0.2 -2.8 +0.2
growth on ypd YPD -0.4 +0.3 -0.2 -0.7 +0.2
growth on ricinoleic acid YNB Glucose +0.4 +0.5 +0.6 -2.7 +0.7
ypd buoyancy 40 hrs Low Buoyancy -0.4 -0.0 +0.4 -0.5 +0.2
ypd buoyancy 40 hrs High Buoyancy -0.4 -0.1 -0.0 +0.9 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.0 -0.1 +0.7 +0.0
auxotrophy Dropout Complete +0.2 +0.2 +0.6 -1.5 +1.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.9 +0.6 +1.0 -2.8 +1.6
growth on ricinoleic acid YNB Methylricinoleic Acid +1.1 +0.7 +1.2 -2.0 +1.2
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