Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14856 and 14857 are separated by 394 nucleotides14857 and 14858 are separated by 359 nucleotides14858 and 14859 are separated by 291 nucleotides14859 and 14860 are separated by 621 nucleotides mRNA_6488: 14856 - K01655 LYS21, LYS20 homocitrate synthase, at 905,172 to 907,549 14856 mRNA_6489: 14857 - KOG0327 Translation initiation factor 4F, helicase subunit (eIF-4A) and related helicases, at 907,944 to 911,303 14857 mRNA_6490: 14858 - KOG1698 Mitochondrial/chloroplast ribosomal protein L19, at 911,663 to 912,456 14858 mRNA_6491: 14859 - KOG2273 Membrane coat complex Retromer, subunit VPS5/SNX1, Sorting nexins, and related PX domain-containing proteins, at 912,748 to 918,213 14859 mRNA_6492: 14860 - KOG3269 Predicted membrane protein, at 918,835 to 919,868 14860
Group Condition mRNA_6488 mRNA_6489 mRNA_6490 mRNA_6491 mRNA_6492
14856 14857 14858 14859 14860
facs on lipid accumulation Sorted Low BODIPY Gate -4.1 +0.3 N.D. +0.2 -0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy -3.2 -0.0 N.D. -0.0 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy -3.3 -0.1 N.D. +0.1 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -2.8 +0.2 N.D. +0.0 -0.9
auxotrophy YNB Glucose -3.9 +0.9 N.D. +0.0 -0.2
auxotrophy YNB Glucose 75mM Arginine -4.3 +0.9 N.D. +0.1 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -3.2 +0.3 N.D. +0.0 -0.2
auxotrophy YNB Glucose 75mM Methionine -4.3 +0.9 N.D. +0.0 +0.4
facs on lipid accumulation Sorted No BODIPY Gate -3.9 +0.2 N.D. +0.2 +0.6
facs on lipid accumulation Sorted High BODIPY Gate -3.1 +0.2 N.D. +0.1 -0.0
growth on oleic acid YNB Glucose -3.8 +0.8 N.D. +0.1 +0.1
growth on ricinoleic acid YNB Glucose -2.7 +0.7 N.D. -0.2 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -2.8 +1.6 N.D. +0.0 -0.9
ypd buoyancy 40 hrs No Buoyancy Separation -1.0 -0.7 N.D. -0.3 +0.2
growth on oleic acid YNB Oleic Acid -2.9 +1.0 N.D. +0.3 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -2.0 +1.2 N.D. +0.1 -0.3
growth on ypd YPD -0.7 +0.2 N.D. +0.1 +0.2
ypd buoyancy 40 hrs Low Buoyancy -0.5 +0.2 N.D. +0.1 +0.1
auxotrophy Dropout Complete -1.5 +1.0 N.D. +0.3 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.7 +0.0 N.D. -0.1 -0.2
ypd buoyancy 40 hrs High Buoyancy +0.9 -0.1 N.D. +0.1 -0.2
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