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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
14856 and 14857 are separated by 394 nucleotides
14857 and 14858 are separated by 359 nucleotides
14858 and 14859 are separated by 291 nucleotides
14859 and 14860 are separated by 621 nucleotides
mRNA_6488: 14856 - K01655 LYS21, LYS20 homocitrate synthase, at 905,172 to 907,549
14856
mRNA_6489: 14857 - KOG0327 Translation initiation factor 4F, helicase subunit (eIF-4A) and related helicases, at 907,944 to 911,303
14857
mRNA_6490: 14858 - KOG1698 Mitochondrial/chloroplast ribosomal protein L19, at 911,663 to 912,456
14858
mRNA_6491: 14859 - KOG2273 Membrane coat complex Retromer, subunit VPS5/SNX1, Sorting nexins, and related PX domain-containing proteins, at 912,748 to 918,213
14859
mRNA_6492: 14860 - KOG3269 Predicted membrane protein, at 918,835 to 919,868
14860
Group
Condition
mRNA
_6488
mRNA
_6489
mRNA
_6490
mRNA
_6491
mRNA
_6492
14856
14857
14858
14859
14860
facs on lipid accumulation
Sorted Low BODIPY Gate
-4.1
+0.3
N.D.
+0.2
-0.6
buoyancy on lipid accumulation
40 hrs High Buoyancy
-3.2
-0.0
N.D.
-0.0
-0.5
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-3.3
-0.1
N.D.
+0.1
-0.3
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-2.8
+0.2
N.D.
+0.0
-0.9
auxotrophy
YNB Glucose
-3.9
+0.9
N.D.
+0.0
-0.2
auxotrophy
YNB Glucose 75mM Arginine
-4.3
+0.9
N.D.
+0.1
+0.3
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-3.2
+0.3
N.D.
+0.0
-0.2
auxotrophy
YNB Glucose 75mM Methionine
-4.3
+0.9
N.D.
+0.0
+0.4
facs on lipid accumulation
Sorted No BODIPY Gate
-3.9
+0.2
N.D.
+0.2
+0.6
facs on lipid accumulation
Sorted High BODIPY Gate
-3.1
+0.2
N.D.
+0.1
-0.0
growth on oleic acid
YNB Glucose
-3.8
+0.8
N.D.
+0.1
+0.1
growth on ricinoleic acid
YNB Glucose
-2.7
+0.7
N.D.
-0.2
-0.1
growth on ricinoleic acid
YNB Ricinoleic Acid
-2.8
+1.6
N.D.
+0.0
-0.9
ypd buoyancy
40 hrs No Buoyancy Separation
-1.0
-0.7
N.D.
-0.3
+0.2
growth on oleic acid
YNB Oleic Acid
-2.9
+1.0
N.D.
+0.3
-0.1
growth on ricinoleic acid
YNB Methylricinoleic Acid
-2.0
+1.2
N.D.
+0.1
-0.3
growth on ypd
YPD
-0.7
+0.2
N.D.
+0.1
+0.2
ypd buoyancy
40 hrs Low Buoyancy
-0.5
+0.2
N.D.
+0.1
+0.1
auxotrophy
Dropout Complete
-1.5
+1.0
N.D.
+0.3
+0.2
ypd buoyancy
40 hrs Median Buoyancy
+0.7
+0.0
N.D.
-0.1
-0.2
ypd buoyancy
40 hrs High Buoyancy
+0.9
-0.1
N.D.
+0.1
-0.2
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