Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14857 and 14858 are separated by 359 nucleotides14858 and 14859 are separated by 291 nucleotides14859 and 14860 are separated by 621 nucleotides14860 and 14861 are separated by 156 nucleotides mRNA_6489: 14857 - KOG0327 Translation initiation factor 4F, helicase subunit (eIF-4A) and related helicases, at 907,944 to 911,303 14857 mRNA_6490: 14858 - KOG1698 Mitochondrial/chloroplast ribosomal protein L19, at 911,663 to 912,456 14858 mRNA_6491: 14859 - KOG2273 Membrane coat complex Retromer, subunit VPS5/SNX1, Sorting nexins, and related PX domain-containing proteins, at 912,748 to 918,213 14859 mRNA_6492: 14860 - KOG3269 Predicted membrane protein, at 918,835 to 919,868 14860 mRNA_6493: 14861 - K20301 TRAPPC2, TRS20 trafficking protein particle complex subunit 2, at 920,025 to 920,861 14861
Group Condition mRNA_6489 mRNA_6490 mRNA_6491 mRNA_6492 mRNA_6493
14857 14858 14859 14860 14861
ypd buoyancy 40 hrs No Buoyancy Separation -0.7 N.D. -0.3 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. +0.0 -0.9 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 N.D. -0.0 -0.5 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. +0.1 -0.3 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. +0.1 -0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. -0.1 -0.2 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 N.D. +0.2 -0.6 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 N.D. +0.0 -0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.2 N.D. +0.1 -0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. +0.1 +0.1 N.D.
growth on ricinoleic acid YNB Glucose +0.7 N.D. -0.2 -0.1 N.D.
growth on ypd YPD +0.2 N.D. +0.1 +0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +1.6 N.D. +0.0 -0.9 N.D.
auxotrophy YNB Glucose +0.9 N.D. +0.0 -0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +1.2 N.D. +0.1 -0.3 N.D.
growth on oleic acid YNB Glucose +0.8 N.D. +0.1 +0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. +0.2 +0.6 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.9 N.D. +0.1 +0.3 N.D.
growth on oleic acid YNB Oleic Acid +1.0 N.D. +0.3 -0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.9 N.D. +0.0 +0.4 N.D.
auxotrophy Dropout Complete +1.0 N.D. +0.3 +0.2 N.D.
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