Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14863 and 14864 are separated by 265 nucleotides14864 and 14865 are separated by 90 nucleotides14865 and 14866 are separated by 147 nucleotides14866 and 14867 are separated by 53 nucleotides mRNA_6495: 14863 - Hypothetical Protein, at 925,916 to 926,829 14863 mRNA_6496: 14864 - HMMPfam-Fungal protein of unknown function (DUF1748)-PF08520, at 927,095 to 927,579 14864 mRNA_6497: 14865 - HMMPfam-Peptidase family M28-PF04389,SUPERFAMILY--SSF53187, at 927,670 to 929,364 14865 mRNA_6498: 14866 - K00226 pyrD dihydroorotate dehydrogenase (fumarate), at 929,512 to 931,049 14866 mRNA_6499: 14867 - K12668 OST2, DAD1 oligosaccharyltransferase complex subunit epsilon, at 931,103 to 931,809 14867
Group Condition mRNA_6495 mRNA_6496 mRNA_6497 mRNA_6498 mRNA_6499
14863 14864 14865 14866 14867
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. +0.4 -3.7 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.8 N.D. +0.0 -0.3 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 N.D. -0.1 -0.3 N.D.
growth on ypd YPD +0.2 N.D. -0.0 -0.7 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.7 N.D. +0.1 +0.0 N.D.
growth on oleic acid YNB Oleic Acid -0.0 N.D. -0.1 -0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.4 N.D. +0.1 -0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. -0.1 -0.5 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. +0.3 -0.8 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.4 N.D. +0.2 +0.0 N.D.
auxotrophy YNB Glucose +0.5 N.D. -0.2 -0.5 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. -0.1 +0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. +0.1 -0.1 N.D.
growth on oleic acid YNB Glucose +0.2 N.D. +0.1 -0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. +0.2 -0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. -0.0 +0.1 N.D.
auxotrophy Dropout Complete +0.5 N.D. +0.2 -0.4 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 N.D. -0.1 +0.4 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.3 N.D. +0.0 +0.3 N.D.
growth on ricinoleic acid YNB Glucose +0.2 N.D. +0.1 +0.4 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.5 N.D. +0.2 +0.3 N.D.
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