Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14864 and 14865 are separated by 90 nucleotides14865 and 14866 are separated by 147 nucleotides14866 and 14867 are separated by 53 nucleotides14867 and 14868 are separated by 1812 nucleotides mRNA_6496: 14864 - HMMPfam-Fungal protein of unknown function (DUF1748)-PF08520, at 927,095 to 927,579 14864 mRNA_6497: 14865 - HMMPfam-Peptidase family M28-PF04389,SUPERFAMILY--SSF53187, at 927,670 to 929,364 14865 mRNA_6498: 14866 - K00226 pyrD dihydroorotate dehydrogenase (fumarate), at 929,512 to 931,049 14866 mRNA_6499: 14867 - K12668 OST2, DAD1 oligosaccharyltransferase complex subunit epsilon, at 931,103 to 931,809 14867 mRNA_6500: 14868 - K14416 HBS1 elongation factor 1 alpha-like protein, at 933,622 to 935,186 14868
Group Condition mRNA_6496 mRNA_6497 mRNA_6498 mRNA_6499 mRNA_6500
14864 14865 14866 14867 14868
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.4 -3.7 N.D. +0.9
auxotrophy YNB Glucose N.D. -0.2 -0.5 N.D. -1.2
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 -0.5 N.D. -1.0
auxotrophy YNB Glucose 75mM Methionine N.D. +0.3 -0.8 N.D. -0.7
growth on oleic acid YNB Oleic Acid N.D. -0.1 -0.4 N.D. -0.5
auxotrophy Dropout Complete N.D. +0.2 -0.4 N.D. -0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.1 +0.1 N.D. -0.9
growth on ypd YPD N.D. -0.0 -0.7 N.D. -0.1
growth on ricinoleic acid YNB Glucose N.D. +0.1 +0.4 N.D. -1.3
growth on oleic acid YNB Glucose N.D. +0.1 -0.2 N.D. -0.6
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.2 +0.0 N.D. -0.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 -0.3 N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 -0.1 N.D. -0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.0 +0.1 N.D. -0.4
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 -0.3 N.D. -0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.1 +0.4 N.D. -0.6
ypd buoyancy 40 hrs High Buoyancy N.D. +0.0 +0.3 N.D. -0.6
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.0 -0.3 N.D. +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.2 +0.3 N.D. -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.2 -0.0 N.D. +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 +0.0 N.D. +0.2
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