Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14866 and 14867 are separated by 53 nucleotides14867 and 14868 are separated by 1812 nucleotides14868 and 14869 are separated by 238 nucleotides14869 and 14870 are separated by 316 nucleotides mRNA_6498: 14866 - K00226 pyrD dihydroorotate dehydrogenase (fumarate), at 929,512 to 931,049 14866 mRNA_6499: 14867 - K12668 OST2, DAD1 oligosaccharyltransferase complex subunit epsilon, at 931,103 to 931,809 14867 mRNA_6500: 14868 - K14416 HBS1 elongation factor 1 alpha-like protein, at 933,622 to 935,186 14868 mRNA_6501: 14869 - K06902 UMF1 MFS transporter, UMF1 family, at 935,425 to 937,523 14869 mRNA_6502: 14870 - K02971 RP-S21e, RPS21 small subunit ribosomal protein S21e, at 937,840 to 938,370 14870
Group Condition mRNA_6498 mRNA_6499 mRNA_6500 mRNA_6501 mRNA_6502
14866 14867 14868 14869 14870
ypd buoyancy 40 hrs No Buoyancy Separation -3.7 N.D. +0.9 -0.0 N.D.
auxotrophy YNB Glucose -0.5 N.D. -1.2 +0.0 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.8 N.D. -0.7 +0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.5 N.D. -1.0 +0.3 N.D.
growth on ypd YPD -0.7 N.D. -0.1 -0.4 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. -0.9 -0.3 N.D.
auxotrophy Dropout Complete -0.4 N.D. -0.7 +0.1 N.D.
growth on ricinoleic acid YNB Glucose +0.4 N.D. -1.3 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 N.D. -0.1 -0.4 N.D.
growth on oleic acid YNB Oleic Acid -0.4 N.D. -0.5 +0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.0 N.D. -0.8 -0.1 N.D.
growth on oleic acid YNB Glucose -0.2 N.D. -0.6 +0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.3 N.D. -0.2 +0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. -0.4 +0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 N.D. -0.4 -0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 N.D. -0.6 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 N.D. +0.2 -0.4 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. +0.0 -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. -0.4 +0.3 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.3 N.D. -0.6 +0.4 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 N.D. +0.2 +0.3 N.D.
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