Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14870 and 14871 are separated by 640 nucleotides14871 and 14872 are separated by 391 nucleotides14872 and 14873 are separated by 429 nucleotides14873 and 14874 are separated by 408 nucleotides mRNA_6502: 14870 - K02971 RP-S21e, RPS21 small subunit ribosomal protein S21e, at 937,840 to 938,370 14870 mRNA_6503: 14871 - K11360 SPT8 transcriptional activator SPT8, at 939,011 to 940,996 14871 mRNA_6504: 14872 - Hypothetical Protein, at 941,388 to 943,563 14872 mRNA_6505: 14873 - Hypothetical Protein, at 943,993 to 944,857 14873 mRNA_6506: 14874 - KOG2712 Transcriptional coactivator, at 945,266 to 946,046 14874
Group Condition mRNA_6502 mRNA_6503 mRNA_6504 mRNA_6505 mRNA_6506
14870 14871 14872 14873 14874
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.4 -0.7 -0.2 -1.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.5 +0.1 -0.2 -0.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.3 +0.2 -0.6 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 -0.0 -0.2 -0.5
auxotrophy YNB Glucose 75mM Arginine N.D. -0.4 +0.0 +0.0 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.0 +0.3 -0.2 -0.4
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 +0.2 -0.3 -0.2
growth on oleic acid YNB Glucose N.D. +0.1 -0.1 +0.2 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 +0.3 -0.4 -0.1
auxotrophy YNB Glucose N.D. +0.2 -0.1 -0.0 -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.2 +0.0 +0.3 -0.6
auxotrophy Dropout Complete N.D. +0.1 -0.0 +0.2 -0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 +0.2 +0.1 -0.3
growth on ypd YPD N.D. +0.0 -0.0 -0.0 +0.4
growth on oleic acid YNB Oleic Acid N.D. +0.6 -0.4 -0.1 +0.2
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 +0.2 +0.0 +0.3
growth on ricinoleic acid YNB Glucose N.D. -0.3 +0.2 -0.2 +0.9
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.6 -0.1 +0.1 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.6 -0.1 -0.1 +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.0 +0.1 -0.0 +1.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.2 -0.1 -0.4 +1.8
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