Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14876 and 14877 are separated by 159 nucleotides14877 and 14878 are separated by 195 nucleotides14878 and 14879 are separated by 142 nucleotides14879 and 14880 are separated by 700 nucleotides mRNA_6508: 14876 - KOG2688 Transcription-associated recombination protein - Thp1p, at 948,399 to 950,118 14876 mRNA_6509: 14877 - K12494 PSD PH and SEC7 domain-containing protein, at 950,278 to 955,327 14877 mRNA_6510: 14878 - K00818 E2.6.1.11, argD acetylornithine aminotransferase, at 955,523 to 957,319 14878 mRNA_6511: 14879 - KOG1061 Vesicle coat complex AP-1/AP-2/AP-4, beta subunit, at 957,462 to 960,234 14879 mRNA_6512: 14880 - KOG1089 Myotubularin-related phosphatidylinositol 3-phosphate 3-phosphatase MTM6, at 960,935 to 963,520 14880
Group Condition mRNA_6508 mRNA_6509 mRNA_6510 mRNA_6511 mRNA_6512
14876 14877 14878 14879 14880
auxotrophy YNB Glucose N.D. +0.0 -1.5 N.D. -0.4
auxotrophy YNB Glucose 75mM Methionine N.D. -0.1 -1.1 N.D. -0.6
growth on ricinoleic acid YNB Glucose N.D. +0.1 -1.5 N.D. -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.2 -1.0 N.D. +0.0
growth on oleic acid YNB Glucose N.D. +0.0 -1.1 N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.0 -0.7 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 -0.7 N.D. -0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 -0.4 N.D. -0.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.0 -0.7 N.D. +0.1
auxotrophy Dropout Complete N.D. +0.0 -0.0 N.D. -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.2 -0.1 N.D. -0.5
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 +0.1 N.D. -0.6
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.2 -0.3 N.D. +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.4 -1.0 N.D. +0.3
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 +0.1 N.D. -0.1
growth on ypd YPD N.D. +0.1 -0.3 N.D. +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 +0.1 N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 +0.1 N.D. -0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.2 +0.2 N.D. +0.2
growth on oleic acid YNB Oleic Acid N.D. +0.2 +0.3 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.3 +0.3 N.D. +0.2
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