Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14878 and 14879 are separated by 142 nucleotides14879 and 14880 are separated by 700 nucleotides14880 and 14881 are separated by 1386 nucleotides14881 and 14882 are separated by 968 nucleotides mRNA_6510: 14878 - K00818 E2.6.1.11, argD acetylornithine aminotransferase, at 955,523 to 957,319 14878 mRNA_6511: 14879 - KOG1061 Vesicle coat complex AP-1/AP-2/AP-4, beta subunit, at 957,462 to 960,234 14879 mRNA_6512: 14880 - KOG1089 Myotubularin-related phosphatidylinositol 3-phosphate 3-phosphatase MTM6, at 960,935 to 963,520 14880 mRNA_6513: 14881 - HMMPfam-Glycosyl transferase family 90-PF05686,SMART-Putative lipopolysaccharide-modifying enzyme.-SM00672, at 964,907 to 967,353 14881 mRNA_6514: 14882 - KOG1441 Glucose-6-phosphate/phosphate and phosphoenolpyruvate/phosphate antiporter, at 968,322 to 969,938 14882
Group Condition mRNA_6510 mRNA_6511 mRNA_6512 mRNA_6513 mRNA_6514
14878 14879 14880 14881 14882
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 N.D. +0.2 -1.4 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy -1.0 N.D. +0.0 -0.8 -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.7 N.D. +0.1 -0.6 -0.0
growth on ricinoleic acid YNB Glucose -1.5 N.D. -0.4 +0.8 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.7 N.D. -0.2 +0.4 -0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.7 N.D. -0.1 +0.3 -0.4
auxotrophy YNB Glucose 75mM Methionine -1.1 N.D. -0.6 +0.4 +0.4
auxotrophy YNB Glucose -1.5 N.D. -0.4 +0.6 +0.4
facs on lipid accumulation Sorted High BODIPY Gate -1.0 N.D. +0.3 +0.6 -0.7
growth on oleic acid YNB Glucose -1.1 N.D. +0.1 +0.7 -0.5
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. +0.2 -0.5 -0.4
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. -0.1 -0.4 -0.0
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. -0.0 -0.5 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. -0.1 +0.7 -0.7
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. -0.6 +0.4 +0.1
auxotrophy Dropout Complete -0.0 N.D. -0.5 +1.0 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 N.D. -0.4 +1.9 -0.1
growth on ypd YPD -0.3 N.D. +0.2 +0.4 +0.7
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. -0.5 +1.8 +0.0
ypd buoyancy 40 hrs Low Buoyancy +0.3 N.D. +0.2 +1.5 -0.1
growth on oleic acid YNB Oleic Acid +0.3 N.D. +0.1 +1.3 +0.3
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