Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14883 and 14884 are separated by 344 nucleotides14884 and 14885 are separated by 262 nucleotides14885 and 14886 are separated by 123 nucleotides14886 and 14887 are separated by 291 nucleotides mRNA_6515: 14883 - K16578 CLASP1_2 CLIP-associating protein 1/2, at 970,088 to 973,817 14883 mRNA_6516: 14884 - Hypothetical Protein, at 974,162 to 975,105 14884 mRNA_6517: 14885 - K12879 THOC2 THO complex subunit 2, at 975,368 to 982,291 14885 mRNA_6518: 14886 - K05712 mhpA 3-(3-hydroxy-phenyl)propionate hydroxylase, at 982,415 to 984,966 14886 mRNA_6519: 14887 - K11806 WDSOF1 WD repeat and SOF domain-containing protein 1, at 985,258 to 987,431 14887
Group Condition mRNA_6515 mRNA_6516 mRNA_6517 mRNA_6518 mRNA_6519
14883 14884 14885 14886 14887
growth on ypd YPD +0.2 -1.6 N.D. +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.2 -0.6 N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.6 N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 -0.9 N.D. +0.0 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.2 N.D. -0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.0 N.D. -0.0 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.1 N.D. -0.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 +0.3 N.D. -0.0 N.D.
growth on oleic acid YNB Glucose +0.0 +0.1 N.D. -0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.2 +0.6 N.D. -0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.3 N.D. +0.2 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.0 -0.1 N.D. +0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.0 +0.0 N.D. +0.2 N.D.
auxotrophy YNB Glucose +0.2 +0.0 N.D. +0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.3 N.D. -0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.2 N.D. -0.1 N.D.
growth on oleic acid YNB Oleic Acid +0.2 -0.0 N.D. +0.1 N.D.
growth on ricinoleic acid YNB Glucose +0.3 +0.0 N.D. +0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.5 N.D. -0.0 N.D.
auxotrophy Dropout Complete +0.0 +0.2 N.D. +0.4 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 +1.1 N.D. +0.1 N.D.
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